G蛋白偶联雌激素受体1(GPER)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for G Protein Coupled Estrogen Receptor 1 (GPER)

GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1

  • G蛋白偶联雌激素受体1(GPER)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • G蛋白偶联雌激素受体1(GPER)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • G蛋白偶联雌激素受体1(GPER)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEG045Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测G蛋白偶联雌激素受体1(GPER)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将G蛋白偶联雌激素受体1(GPER)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的G蛋白偶联雌激素受体1(GPER)与连接于固相载体上的抗体结合，然后加入生物素化的G蛋白偶联雌激素受体1(GPER)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的G蛋白偶联雌激素受体1(GPER)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPG045Mu01 G蛋白偶联雌激素受体1(GPER)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPG045Mu02 G蛋白偶联雌激素受体1(GPER)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAG045Mu01 G蛋白偶联雌激素受体1(GPER)多克隆抗体 WB; IHC; ICC; IP.
PAG045Mu02 G蛋白偶联雌激素受体1(GPER)多克隆抗体 WB; IHC; ICC; IP.
SEG045Mu G蛋白偶联雌激素受体1(GPER)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG045Mu G蛋白偶联雌激素受体1(GPER)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
The Journal of Steroid Biochemistry and Molecular Biology Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling [Pubmed:24846829]
Molecular and Cellular Endocrinology Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells [Pubmed:24859647]
J Steroid Biochem Mol Biol Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR32 signalling [PubMed: 24846831]
Journal of Environmental Sciences Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts [science:S1001074216300924]
Psychiatry research Serum levels of G protein-coupled estrogen receptor 1 (GPER1) in drug-naive patients with generalized anxiety disorder. [pubmed:27512921]
Clinical Psychopharmacology Neuroscience. Increased Serum G Protein-coupled Estrogen Receptor 1 Levels and Its Diagnostic Value in Drug Naïve Patients with Major Depressive Disorder [pubmed:29073745]
Psychiatry Research Decreased levels of G protein-coupled estrogen receptor in children with autism spectrum disorders. [pubmed:28734238]
Neuropsychiatric disease and treatment serum levels of gPer-1 in euthymic bipolar patients [Pubmed:29618927]
European archives of oto-rhino-laryngology An evaluation of G-protein coupled membrane estrogen receptor-1 level in stuttering [Pubmed:29299745]
Bosnian Journal of Basic Medical Sciences Evaluation of estrogen and G protein-coupled estrogen receptor 1 (GPER) levels in drug-naïve patients with attention deficit hyperactivity disorder (ADHD) [Pubmed:29659348]
Carleton University Research Virtual Environment In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts []
Am J Otolaryngol GPER-1 and sex-hormone levels in patients with otosclerosis [Pubmed: 32144019]
Andrologia. Relationship between the G protein–coupled oestrogen receptor and spermatogenesis, and its correlation with male infertility [Pubmed: 32776559]
Journal of Radiation Research and Applied Sciences GPER-1 in chronic hepatitis B []
FASEB J Endocrine disruption in Crohn's disease: Bisphenol A enhances systemic inflammatory response in patients with gut barrier translocation of dysbiotic microbiota?¡­ [34085740]
Comparing the Impact of Serum GPER-1 and Oxidant/Antioxidant Levels on Retinopathy in Diabetic Patients and Healthy Individuals: A Pilot Study []
Horm Mol Biol Clin Investig The change pattern in serum G protein-coupled estrogen receptor-1 (GPER1) levels during pregnancy with and without gestational diabetes mellitus [34787384]
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