甘油醛-3-磷酸脱氢酶(GAPDH)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH

  • 甘油醛-3-磷酸脱氢酶(GAPDH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 甘油醛-3-磷酸脱氢酶(GAPDH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 甘油醛-3-磷酸脱氢酶(GAPDH)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB932Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测甘油醛-3-磷酸脱氢酶(GAPDH),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将甘油醛-3-磷酸脱氢酶(GAPDH)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的甘油醛-3-磷酸脱氢酶(GAPDH)与连接于固相载体上的抗体结合,然后加入生物素化的甘油醛-3-磷酸脱氢酶(GAPDH)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的甘油醛-3-磷酸脱氢酶(GAPDH)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB932Mu02 甘油醛-3-磷酸脱氢酶(GAPDH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB932Mu01 甘油醛-3-磷酸脱氢酶(GAPDH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB932Mu01 甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体 WB; IHC; ICC; IP.
PAB932Mu02 甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体 WB; IHC; ICC; IP.
LAB932Mu71 甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB932Mu 甘油醛-3-磷酸脱氢酶(GAPDH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Clin Cancer Res Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402]
Clin Cancer Res Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402]
Diabetes & Metabolism Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells. [pubmed:28499695]
Scientific Reports Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression [pubmed:28720868]
Journal of Biological Chemistry Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167]
Biomaterials Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels [Pubmed: 30343256]
Bulletin of Experimental Biology and Medicine Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells [Pubmed: 30488202]
Journal of Medicinal Chemistry Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral Inhibitors [Pubmed: 30938999]
International Immunopharmacology Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway [Pubmed: 31078923]
Cancers Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia [Pubmed: 31718075]
Biological research Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … [Pubmed: 32046779]
Biomed Pharmacother Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway [Pubmed: 32028241]
Metab Brain Dis Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats [Pubmed: 33170419]
South African Journal of Botany Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo []
Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice []
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