α1-酸性糖蛋白(a1AGP)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)

AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1

  • α1-酸性糖蛋白(a1AGP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • α1-酸性糖蛋白(a1AGP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • α1-酸性糖蛋白(a1AGP)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA816Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测α1-酸性糖蛋白(a1AGP),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的α1-酸性糖蛋白(a1AGP)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-94 83
EDTA plasma(n=5) 78-104 97
heparin plasma(n=5) 86-104 90

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的α1-酸性糖蛋白(a1AGP),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中α1-酸性糖蛋白(a1AGP)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-89% 86-98% 92-99% 82-103%
EDTA plasma(n=5) 85-92% 85-101% 88-97% 95-104%
heparin plasma(n=5) 80-99% 97-104% 88-103% 80-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将α1-酸性糖蛋白(a1AGP)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的α1-酸性糖蛋白(a1AGP)与连接于固相载体上的抗体结合,然后加入生物素化的α1-酸性糖蛋白(a1AGP)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的α1-酸性糖蛋白(a1AGP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA816Hu01 α1-酸性糖蛋白(a1AGP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA816Hu01 α1-酸性糖蛋白(a1AGP)多克隆抗体 WB
PAA816Hu02 α1-酸性糖蛋白(a1AGP)多克隆抗体 WB; IHC; ICC; IP.
LAA816Hu71 α1-酸性糖蛋白(a1AGP)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA816Hu21 α1-酸性糖蛋白(a1AGP)单克隆抗体 WB; IHC
MAA816Hu22 α1-酸性糖蛋白(a1AGP)单克隆抗体 WB,IHC
MAA816Hu23 α1-酸性糖蛋白(a1AGP)单克隆抗体 WB; IHC; ICC; IP.
LAA816Hu72 α1-酸性糖蛋白(a1AGP)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA816Hu α1-酸性糖蛋白(a1AGP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA816Hu α1-酸性糖蛋白(a1AGP)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA816Hu α1-酸性糖蛋白(a1AGP)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSA816Hu01 α1-酸性糖蛋白(a1AGP)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA816Hu01 α1-酸性糖蛋白(a1AGP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
J Reprod Dev.? An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro [Pubmed:Pmc4219990]
Molecular Reproduction and Development Evidence for a novel, local acute-phase response in the bovine oviduct: Progesterone and lipopolysaccharide up-regulate alpha 1-acid-glycoprotein expression in epithelial cells in vitro [Pubmed:25123565]
BMC Complementary and Alternative Medicine Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121]
PLoS One Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845]
Sci Rep.  S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine [pmc:PMC4810364]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
Scientific Reports An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle [pubmed:28667317]
33 Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes. [pubmed:28927749]
Brazilian Journal of Otorhinolaryngology Inflammatory markers in palatine tonsils of children with obstructive sleep apnea syndrome [Pubmed: 30213594]
BioRxiv Proteome-wide characterization and biomarker identification of intracranial aneurysm []
The FEBS journal Dramatically changed immune‐related molecules as early diagnostic biomarkers of non‐small cell lung cancer [Pubmed: 31482685]
PLoS One Effects of vaccination on acute-phase protein response in broiler chicken [Pubmed: 32191767]
Indian Journal of Animal Sciences Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy … []
A New Biomarker Tool for Risk Stratification in ¡°De Novo¡± Acute Heart Failure (OROME) []
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