血管紧张素转化酶(ACE)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Angiotensin I Converting Enzyme (ACE)

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

  • 血管紧张素转化酶(ACE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管紧张素转化酶(ACE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管紧张素转化酶(ACE)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA004Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管紧张素转化酶(ACE),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管紧张素转化酶(ACE)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 84-94 88
EDTA plasma(n=5) 86-93 90
heparin plasma(n=5) 86-103 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的血管紧张素转化酶(ACE),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中血管紧张素转化酶(ACE)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 87-94% 78-101% 80-91%
EDTA plasma(n=5) 94-102% 89-98% 98-105% 78-94%
heparin plasma(n=5) 84-95% 90-97% 99-105% 93-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将血管紧张素转化酶(ACE)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的血管紧张素转化酶(ACE)与连接于固相载体上的抗体结合,然后加入生物素化的血管紧张素转化酶(ACE)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管紧张素转化酶(ACE)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA004Hu01 血管紧张素转化酶(ACE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA004Hu02 血管紧张素转化酶(ACE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA004Hu02 血管紧张素转化酶(ACE)活性蛋白 Cell culture; Activity Assays.
PAA004Hu01 血管紧张素转化酶(ACE)多克隆抗体 WB; IHC
PAA004Hu02 血管紧张素转化酶(ACE)多克隆抗体 WB; ICC; IHC-P; IHC-F; ELISA.
LAA004Hu81 血管紧张素转化酶(ACE)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA004Hu71 血管紧张素转化酶(ACE)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA004Hu22 血管紧张素转化酶(ACE)单克隆抗体 WB; IHC; ICC; IP.
MAA004Hu21 血管紧张素转化酶(ACE)单克隆抗体 WB; IHC; ICC; IP.
SEA004Hu 血管紧张素转化酶(ACE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA004Hu 血管紧张素转化酶(ACE)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA004Hu 血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Lipids in Health and disease Association of elevated blood pressure and impaired vasorelaxation in experimental Sprague-Dawley rats fed with heated vegetable oil [PubMed: PMC2914008]
Malaysian Journal of Medical Sciences Effect of Repeatedly Heated Palm Olein on Blood Pressure–Regulating Enzymes Activity and Lipid Peroxidation in Rats [Usm: MJMS19120]
Journal of Cancer Therapy Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source]
The American journal of clinical nutrition Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial. [Pubmed: 23553151]
Journal of Vascular Surgery Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice [Pubmed:25037606]
Reprod Toxicol.? ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467]
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
Chem Biol Interact Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling [PubMed: 26079204]
Biomed Res Int Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress [PubMed: 26640794]
Clinics (Sao Paulo) Mechanisms [PubMed: 26602523]
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
Intensive Care Medicine Experimental Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3]
Evid Based Complement Alternat Med. Garlic Attenuates Plasma and Kidney ACE-1 and AngII Modulations in Early Streptozotocin-Induced Diabetic Rats: Renal Clearance and Blood Pressure Implications [Pubmed:27293465]
Вавиловский журнал генетики и селекции Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией [30_Dubinina.pdf]
Animal Biology The effect of heat stress on bull sperm quality and related HSPs expression [content:journals]
biomedicine & pharmacotherapy Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636]
Biomedicine & Pharmacotherapy Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester [pubmed:28992471]
Journal of Functional Foods Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033]
Molecules Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet [Pubmed:29614743]
Journal of Surgical Research Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841]
Intensive Care Medicine Angiotensin-converting enzymes in acute respiratory distress syndrome []
International Journal of Biological Sciences Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction Through AA Metabolism Modulated CYPs Pathway []
Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
American Journal of Respiratory and Critical Care Medicine Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511]
Int Immunopharmacol Crocin protects against cardiotoxicity induced by doxorubicin through TLR-2/NF-κB signal pathway in vivo and vitro [Pubmed: 32388215]
Food Bioscience Djulis (Chenopodium formosanum) and its bioactive compounds affect vasodilation, angiotensin converting enzyme activity, and hypertension []
Journal of Extracellular Vesicles MiR155-5p in adventitial fibroblasts-derived extracellular vesicles inhibits vascular smooth muscle cell proliferation via suppressing angiotensin-converting enzyme … []
Biotechnology and Pharmacy Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats []
Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum … []
留言咨询