连环蛋白β1(β-catenin)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Beta Catenin (β-catenin)
CTNNb1; bCat; b-Cat; CTNN-B1; Catenin Beta 1; Cadherin-Associated Protein
- 编号SEB021Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于5.9pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测连环蛋白β1(β-catenin)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将连环蛋白β1(β-catenin)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的连环蛋白β1(β-catenin)与连接于固相载体上的抗体结合，然后加入生物素化的连环蛋白β1(β-catenin)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的连环蛋白β1(β-catenin)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB021Hu01 | 连环蛋白β1(β-catenin)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB021Hu02 | 连环蛋白β1(β-catenin)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB021Hu01 | 连环蛋白β1(β-catenin)多克隆抗体 | WB; IHC; ICC; IP. |
| CAB021Hu01 | 抗连环蛋白β1(β-catenin)多克隆抗体 | Loading Control of WB. |
| MAB021Hu23 | 连环蛋白β1(β-catenin)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB021Hu24 | 连环蛋白β1(β-catenin)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB021Hu25 | 连环蛋白β1(β-catenin)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB021Hu27 | 连环蛋白β1(β-catenin)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB021Hu21 | 连环蛋白β1(β-catenin)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB021Hu22 | 连环蛋白β1(β-catenin)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB021Hu26 | 连环蛋白β1(β-catenin)单克隆抗体 | WB; IHC; ICC; IP. |
| CAB021Hu22 | 抗连环蛋白β1(β-catenin)单克隆抗体 | Loading Control of WB |
| MAB021Hu29 | 连环蛋白β1(β-catenin)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB021Hu | 连环蛋白β1(β-catenin)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB021Hu | 连环蛋白β1(β-catenin)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB021Hu01 | 连环蛋白β1(β-catenin)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Clinica Chimica Acta | Versican and its associated molecules: Potential diagnostic markers for multiple myeloma [Pubmed:25623955] |
| Journal of Bone and Mineral Research | Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth [Pubmed:25639729] |
| Am J Transl Res | Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats [PubMed: 26396666] |
| Scientific Reports | Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA. [pubmed:28533512] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618] |
| Scientific Reports | Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA [Pubmed: 28533512] |
| The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer [Doi: 10.15369/sujms.30.359] |
| Advances in Medical Sciences | Urinary procollagen III aminoterminal propeptide and β-catenin–New diagnostic biomarkers in solitary functioning kidney? [Pubmed: 30738287] |
| Biomed Pharmacother | Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway [Pubmed: 32028241] |
| Journal of Cosmetic Dermatology | Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling … [Pubmed: 33179848] |
| Nutrients | Hydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia [Pubmed: 32784915] |
| Oncotarget | Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme [33473259] |
| Cytokine | QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model [34564023] |
