瘦素(LEP)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Leptin (LEP)

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

  • 瘦素(LEP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 瘦素(LEP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 瘦素(LEP)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA084Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测瘦素(LEP),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的瘦素(LEP)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 78-90 82
heparin plasma(n=5) 88-104 94

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的瘦素(LEP),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中瘦素(LEP)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 86-101% 99-105% 86-94%
EDTA plasma(n=5) 80-101% 91-101% 90-99% 91-101%
heparin plasma(n=5) 79-101% 85-102% 79-101% 96-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

试剂盒内容

试剂名称 数量 试剂名称 数量
96孔板(预包被) 1 96孔板覆膜 4
标准品 2 标准品稀释液 1×20mL
检测溶液A 1×120µL 检测稀释液A 1×12mL
检测溶液B 1×120µL 检测稀释液B 1×12mL
TMB底物 1×9mL 终止液 1×6mL
浓洗涤液 (30 × concentrate) 1×20mL 说明书 1

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

ELISA Kit for Leptin (LEP)

实验原理

将瘦素(LEP)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的瘦素(LEP)与连接于固相载体上的抗体结合,然后加入生物素化的瘦素(LEP)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的瘦素(LEP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA084Hu01 瘦素(LEP)活性蛋白 Cell culture; Activity Assays.
RPA084Hu01 瘦素(LEP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA084Hu01 瘦素(LEP)多克隆抗体 WB; IHC; ICC; IP.
LAA084Hu71 瘦素(LEP)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA084Hu81 瘦素(LEP)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEA084Hu 瘦素(LEP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA084Hu 瘦素(LEP)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA084Hu01 瘦素(LEP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
European Journal of Pharmacology Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet [PubMed: 20869961]
Indian Journal of Pharmacology Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats [Ijp: source]
Journal of endocrinology Testosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbit [PubMed: 22010203]
PLoS One. Multi-Functional Roles of Chitosan as a Potential Protective Agent against Obesity [PubMed: 23342013]
Molecular and Cellular Endocrinology Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs [ScienceDirect: S0303720712004194]
Journal of Animal and Feed Sciences Ovarian, metabolic and endocrine indexes in dairy cows with different body condition scores [Ifzz: Source]
British Journal of Nutrition n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs [Pubmed: 23947577]
Int J Mol Sci. Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. [Pubmed: 24736781]
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658]
Animal Physiology EFFECT OF LEPTIN GENE ON SERUM LEPTIN CONCENTRATION AND BEEF QUALITY IN CZECH FLECKVIEH BULLS [Mendelu:Source]
Evid Based Complement Alternat Med. Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats [Pubmed:Pmc4363645]
general endocrinology Oxytocin reverse ovariectomy-induced Osteoper and body fat gain [Pubmed:24506069]
Respiratory Physiology &amp; Neurobiology Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring [Pubmed:25500158]
Sci Rep Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis [PubMed: 26634430]
Evid Based Complement Alternat Med Tinospora crispa [PubMed: 25821506]
Ankara Effects of breeder age and early energy restriction on fattening performance, some meat quality traits and plasma leptin concentration of broilers [Dergiler: 11]
MEDICINA VETERIN Comparative measurements of the morphometric pattern and energy profile of Crioula mares in the final third of gestation, with different body condition scores [Scielo: S0102-09352015000300707]
Pancreatic Disorders & Therapy Pancreatic Exocrine Insufficiency Affects Not Only Digestibility of Nutrients and Growth, but Also Body Composition and Endocrinological Parameters – Study on Piglets Used As a Model for Children [Journals: 2165-7092-%20S5-00]
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212]
Animal Anterior pituitary influence on adipokine expression and secretion by porcine adipocytes. [Pubmed:27074715]
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens [journalprintahead:87]
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens [view:87]
Obes Surg. Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. [Pubmed:27185175]
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines [science:S0065128116301234]
J Biomed Mater Res A Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice [Pubmed:26850371]
Clin Epigenetics. The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model [Pubmed:27110299]
International Journal of Molecular Sciences Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency [pubmed:27690005]
Journal of Animal Science Changes in small intestinal morphometry and electrogenic nutrient transport in young pigs with pancreatic exocrine insufficiency [publications:jas]
International Journal of Endocrinology The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages [pubmed:27990162]
journal of animal science The effect of GnRH vaccination on performance, carcass, and meat quality and hormonal regulation in boars, barrows, and gilts. [pubmed:27482668]
journal of animal science Diet-induced modifications to milk composition have long-term effects on offspring growth in rabbits. [pubmed:28380600]
Evidence-Based Complementary and Alternative Medicine Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. [pubmed:28246535]
Asian-Australasian Journal of Animal Sciences Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits [pubmed:27004817]
Stem Cell Research & Therapy Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizingradiation. [pubmed:27530249]
Obesity Surgery Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in ObeseGöttingen Minipig. Effects on Glucose Metabolism. [pubmed:27185175]
Meat Sci Estimation of the intramuscular fat content of m. longissimus thoracis in crossbred beef cattle based on live animal measurements. [pubmed:27940421]
THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY Correlation of Leptin, Insulin and Glucose During Late Gestation and Early Lactation in Murrah Buffaloes [7086]
Journal of Animal and Plant Sciences Effects Of Maca (Lepıdıum Meyenıı Walp) Powder On Serum Indıces And Metabolıc Responses In Racehorses [57cf073008ae83b37462323a.pdf]
留言咨询