基质金属蛋白酶3(MMP3)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Matrix Metalloproteinase 3 (MMP3)

SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1

  • 基质金属蛋白酶3(MMP3)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 基质金属蛋白酶3(MMP3)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 基质金属蛋白酶3(MMP3)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA101Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测基质金属蛋白酶3(MMP3),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的基质金属蛋白酶3(MMP3)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 86-103 93
heparin plasma(n=5) 99-105 102

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的基质金属蛋白酶3(MMP3),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中基质金属蛋白酶3(MMP3)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 81-98% 83-95% 93-103%
heparin plasma(n=5) 90-98% 95-105% 94-105% 88-95%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将基质金属蛋白酶3(MMP3)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的基质金属蛋白酶3(MMP3)与连接于固相载体上的抗体结合,然后加入生物素化的基质金属蛋白酶3(MMP3)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质金属蛋白酶3(MMP3)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
EPA101Hu62 基质金属蛋白酶3(MMP3)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA101Hu01 基质金属蛋白酶3(MMP3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA101Hu02 基质金属蛋白酶3(MMP3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA101Hu04 基质金属蛋白酶3(MMP3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA101Hu03 基质金属蛋白酶3(MMP3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA101Hu61 基质金属蛋白酶3(MMP3)活性蛋白 Cell culture; Activity Assays.
RPA101Hu05 基质金属蛋白酶3(MMP3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA101Hu02 基质金属蛋白酶3(MMP3)多克隆抗体 WB; IHC; ICC; IP.
PAA101Hu01 基质金属蛋白酶3(MMP3)多克隆抗体 WB; IHC; ICC; IP.
PAA101Hu05 基质金属蛋白酶3(MMP3)多克隆抗体 WB; IHC; ICC; IP.
PAA101Hu03 基质金属蛋白酶3(MMP3)多克隆抗体 WB; IHC; ICC; IP.
MAA101Hu22 基质金属蛋白酶3(MMP3)单克隆抗体 WB; IHC; ICC; IP.
MAA101Hu23 基质金属蛋白酶3(MMP3)单克隆抗体 WB; IHC; ICC; IP.
MAA101Hu21 基质金属蛋白酶3(MMP3)单克隆抗体 WB; IHC; ICC; IP.
LAA101Hu72 基质金属蛋白酶3(MMP3)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA101Hu 基质金属蛋白酶3(MMP3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA101Hu 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA101Hu 基质金属蛋白酶3(MMP3)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSA101Hu01 基质金属蛋白酶3(MMP3)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA101Hu01 基质金属蛋白酶3(MMP3)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Journal of Bone and Joint Surgery Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice [PubMed: 21119180]
Infection and Immunity Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier [Pubmed: 23649093]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
China Pharmacy Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats [Source]
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655]
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747]
physiological research The skin remodeling in type 1 diabetes and insulin resistance animal models [PubMed: 26047379]
Physiol Res The skin remodeling in type 1 diabetes and insulin resistance animal models. [PubMed: 26047379]
Cellular & Molecular Immunology Effect of ebosin on modulating interleukin-2&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes [PubMed: 25938977]
Biol Res The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis [PubMed: 26578310]
J Orthop Res. Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. [Pubmed:26748661]
Cellular & Molecular Immunology Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes [pubmed:25938977]
Journal of Orthopaedic Research Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation [pubmed:26748661]
Mediators of Inflammation Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis. [pubmed:27433031]
Molecular Medicine Reports Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells [pubmed:28447732]
der Christian-Albrechts-Universität zu Kiel Wirkung schonender Laserverfahren auf den Komplexaus retinalem Pigmentepithel, Bruch´scher Membranund Choroidea - mögliche Therapieoption der frühen altersabhängigen Makuladegeneration [1133074014:34]
REDVET - Revista electrónica de Veterinaria Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease [E-ISSN: 1695-7504]
REDVET - Revista electrónica de Veterinaria Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease - Medición de los niveles de Metaloproteinasa-3 en liquido sinovial comobiomarcador de enfermedad articular [ISSN 1695-7504]
Molecular Pharmacology Dexamethasone Down-regulates Endothelin Receptors and Reduces Endothelin-induced Production of Matrix Metalloproteinases in Cultured Rat Astrocytes [10.1124/mol.116.107300]
Theranostics Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis [Pubmed:29774078]
Chinese Journal of Integrative Medicine Electroacupuncture Delays Cartilage Degeneration by Modulating Nuclear Factor-κB Signaling Pathway [Pubmed: 30656598]
Effects of a hyperimmunized egg product on voluntary physical activity levels, serum inflammatory markers, and owner perception of joint pain of dogs with?… []
PLoS One Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis [Pubmed: 30939166]
ACS Biomaterials Science & Engineering Fluorogenic Probe for Detecting Active Matrix Metalloproteinase-3 (MMP-3) in Plasma and Peripheral Blood Neutrophils to Indicate the Severity of Rheumatoid … []
World Journal of Pediatrics Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis [Pubmed: 31641939]
International Journal of Molecular Sciences Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin [Pubmed: 31652545]
JOURNAL OF CELLULAR BIOCHEMISTRY Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes [Pubmed: 32162384]
Bone Research Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis [Pubmed: 32047705]
Scientific Reports Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722]
Alexandria Journal for Veterinary Sciences The Clinicopathological Picture of Some Postpartum Disorders in Barki Ewes. []
Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats []
Food Funct Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice [33890588]
journal biomedicine translational research Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic?¡­ []
JOURNAL OF ANIMAL SCIENCE Physical Activity Patterns of Free Living Dogs Diagnosed with Osteoarthritis [34216471]
Osteoarthritis Cartilage A systematic review on the potential value of synovial fluid biomarkers to predict clinical outcomes in cartilage repair treatments [Pubmed:35618204]
Phytomedicine Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK … [Pubmed:35660352]
Journal of Immunology Research Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes [Pubmed:35340587]
留言咨询