内皮型一氧化氮合酶(eNOS)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Endothelial NOS (eNOS)

NOS3; ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS; Nitric Oxide Synthase 3, Endothelial

  • 内皮型一氧化氮合酶(eNOS)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 内皮型一氧化氮合酶(eNOS)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 内皮型一氧化氮合酶(eNOS)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA868Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测内皮型一氧化氮合酶(eNOS),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的内皮型一氧化氮合酶(eNOS)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 95-103 98
heparin plasma(n=5) 87-105 96

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的内皮型一氧化氮合酶(eNOS),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中内皮型一氧化氮合酶(eNOS)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-104% 87-95% 79-98% 96-105%
EDTA plasma(n=5) 80-91% 87-99% 82-90% 81-99%
heparin plasma(n=5) 93-101% 81-105% 92-101% 82-98%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将内皮型一氧化氮合酶(eNOS)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的内皮型一氧化氮合酶(eNOS)与连接于固相载体上的抗体结合,然后加入生物素化的内皮型一氧化氮合酶(eNOS)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的内皮型一氧化氮合酶(eNOS)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA868Hu03 内皮型一氧化氮合酶(eNOS)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA868Hu04 内皮型一氧化氮合酶(eNOS)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA868Hu01 内皮型一氧化氮合酶(eNOS)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA868Hu04 内皮型一氧化氮合酶(eNOS)多克隆抗体 WB; IHC; ICC; IP.
PAA868Hu03 内皮型一氧化氮合酶(eNOS)多克隆抗体 WB; IHC; ICC; IP.
PAA868Hu01 内皮型一氧化氮合酶(eNOS)多克隆抗体 WB; IHC; ICC; IP.
LAA868Hu71 内皮型一氧化氮合酶(eNOS)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA868Hu22 内皮型一氧化氮合酶(eNOS)单克隆抗体 WB; IHC; ICC; IP.
SEA868Hu 内皮型一氧化氮合酶(eNOS)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA868Hu 内皮型一氧化氮合酶(eNOS)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Journal of Ethnopharmacology Impact of matrine on inflammation related factors in rat intestinal microvascular endothelial cells [ScienceDirect: S0378874109004541]
PLoS One A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide [PubMed: 23359814]
6th International Scientific Interdisciplinary Conference (ISIC) Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesity [Knmu: Source]
PLoS One. SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases [Plos: Source]
INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION [Tdmu:Source]
Journal of Clinical Laboratory Analysis Soluble Lectin-Like Oxidized LDL Receptor 1 as a Possible Mediator of Endothelial Dysfunction in Patients With Metabolic Syndrome [Pubmed:24798146]
Endocrine Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia [Pubmed:24840719]
PLOS ONE A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: [PubMed: 25785999]
J Trace Elem Med Biol Comparative angioprotective effects of magnesium compounds [PubMed: 25127069]
Journal of Functional Foods Peptides from hydrolysate of lantern fish (Benthosema pterotum) proved neuroprotective in vitro and in vivo [science:S1756464616300639]
Медицина и здравоохранение Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу [article:n]
journal of molecular and cellular cardiology Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease. [pubmed:28088561]
International Journal of Medicine and Medical Research ThE REmOdELINg Of LEfT ATRIUm ANd vAsOdILATION fAcTORs chANgEs IN IschEmIc hEART fAILURE [7038]
International Journal of Ophthalmology Endothelial nitric oxide synthase deficiency influences normal cell cycle progression andapoptosis in trabecular meshwork cells. [pubmed:27366677]
Biomed Pharmacother. SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. [pubmed:28122311]
Biomedicine & Pharmacotherapy SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death [DOI:10.1016/j.biopha.2017.01.087]
Asian Journal of Science and Technology THE INFLUENCE OF THE ADDITIONAL USAGE OF L-ARGININE ON THE FACTORS OF VASODILATION AND THE CLINICAL DATA, COMPLICATED WITH CARDIAC DECOMPENSATION WITH REDUCED AND PRESERVED FRACTURE OF THE LEFT VENTRICLE THAT PATIENTS WITH STABLE CORONARY HEART DISEASE HAVE [ISSN: 0976-3376]
National Science Review Blunted Nitric Oxide Regulation in Tibetans under High Altitude Hypoxia [10.1093:nsr]
Biomedicine & Pharmacotherapy Molecular basis of vascular damage caused by cigarette smoke exposure and a new approach to the treatment: Alpha-linolenic acid [Pubmed:29579706]
Experimental and Therapeutic Medicine Ergothioneine prevents endothelial dysfunction induced by mercury chloride [Pubmed:29805489]
Turk Kardiyol Dern Ars.  Effect of statins on sirtuin 1 and endothelial nitric oxide synthase expression in young patients with a history of premature myocardial infarction [Pubmed:29664427]
Stężenia śródbłonkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ciężarnych palących tytoń–badania wstępne []
The Eurasian Journal of Medicine Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats [Doi: 10.5152/eurasianjmed.2018.18160]
International Journal of Environmental Research and Public Health Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women [Pubmed: 30513890]
Journal of Physics D: Applied Physics Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility []
Particle and Fibre Toxicology Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathway [Pubmed: 30975181]
ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ … []
Динамика показателей эндотелиальной дисфункции до и после операции у больных варикозной болезнью нижних конечностей []
European Journal of Pharmacology The placental growth factor attenuates intimal hyperplasia in vein grafts by improving endothelial dysfunction [Pubmed: 31836533]
Rheumatol Int Circadian fluctuations of endothelial nitric oxide synthase activity in females with rheumatoid arthritis: a pilot study [Pubmed: 32025851]
Reviews on Recent Clinical Trials Circadian rhythms of endothelial nitric oxide synthase and toll-like receptors 2 production in females with rheumatoid arthritis depending on NOS3 gene polymorphism … [Pubmed: 32297585]
Georgian medical news ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE … [Pubmed: 32242853]
Zaporozhye medical journal Dynamics of endothelial dysfunction indices before and after operation in patients with varicose disease of the lower extremities []
regional hemodynamics and microcirculation Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility []
Оксидативный стресс и эндотелиальная дисфункция в развитии экспериментального радиоиндуцированного повреждения легких и их коррекция … []
Reprod Biol Angiogenic factors measured in aspirated placental tissue between the 10+ 6 and 18+ 3 weeks of gestation [34798517]
J Basic Clin Physiol Pharmacol Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats [Pubmed:34986543]
Eur Rev Med Pharmacol Sci Serum inducible and endothelial nitric oxide synthase in coronary artery disease patients with Type 2 Diabetes mellitus [Pubmed:35647851]
Evidence-Based Complementary and Alternative Medicine Mechanism of Fructus Mume Pills Underlying Their Protective Effects in Rats with Acetic Acid-Inducedulcerative Colitis via the Regulation of Inflammatory … [Pubmed:35620404]
留言咨询