免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Immunoglobulin G (IgG)

  • 免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA544Eq.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测免疫球蛋白G(IgG),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的免疫球蛋白G(IgG)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 82-99 96
EDTA plasma(n=5) 78-101 96
heparin plasma(n=5) 92-101 96

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的免疫球蛋白G(IgG),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中免疫球蛋白G(IgG)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 82-94% 84-103% 78-98%
EDTA plasma(n=5) 89-98% 84-97% 83-97% 97-105%
heparin plasma(n=5) 93-101% 93-105% 90-101% 86-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

试剂盒内容

试剂名称 数量 试剂名称 数量
96孔板(预包被) 1 96孔板覆膜 4
标准品 2 标准品稀释液 1×20mL
检测溶液A 1×120µL 检测稀释液A 1×12mL
检测溶液B 1×120µL 检测稀释液B 1×12mL
TMB底物 1×9mL 终止液 1×6mL
浓洗涤液 (30 × concentrate) 1×20mL 说明书 1

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

ELISA Kit for Immunoglobulin G (IgG)

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将免疫球蛋白G(IgG)单克隆抗体包被微孔板,制成固相载 体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Equus caballus; Equine (Horse,马) 应用(仅供研究使用,不用于临床诊断!)
NPA544Eq01 免疫球蛋白G(IgG)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA544Eq01 免疫球蛋白G(IgG)多克隆抗体 WB; IHC; ICC; IP.
SAA544Eq09 兔抗马IgG多克隆抗体(辣根过氧化物酶标记) WB; IHC; ICC.
SAA544Eq07 兔抗马IgG多克隆抗体(生物素标记) WB; IHC; ICC.
SAA544Eq08 兔抗马IgG多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA544Eq22 免疫球蛋白G(IgG)单克隆抗体 WB; IHC; ICC; IP.
CEA544Eq 免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA544Eq 免疫球蛋白G(IgG)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA544Eq11 免疫球蛋白G(IgG)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

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