白介素1α(IL1a)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Interleukin 1 Alpha (IL1a)

IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1

  • 白介素1α(IL1a)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 白介素1α(IL1a)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 白介素1α(IL1a)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA071Cp.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测白介素1α(IL1a),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的白介素1α(IL1a)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 95-104 98
EDTA plasma(n=5) 96-103 101
heparin plasma(n=5) 80-89 85

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的白介素1α(IL1a),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中白介素1α(IL1a)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 81-98% 81-94% 79-94% 79-92%
EDTA plasma(n=5) 93-101% 80-101% 98-105% 91-101%
heparin plasma(n=5) 87-99% 84-104% 92-101% 85-95%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将白介素1α(IL1a)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的白介素1α(IL1a)与连接于固相载体上的抗体结合,然后加入生物素化的白介素1α(IL1a)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素1α(IL1a)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Capra hircus; Caprine (Goat,山羊) 应用(仅供研究使用,不用于临床诊断!)
APA071Cp61 白介素1α(IL1a)活性蛋白 Cell culture; Activity Assays.
RPA071Cp01 白介素1α(IL1a)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA071Cp02 白介素1α(IL1a)多克隆抗体 WB; IHC; ICC; IP.
PAA071Cp01 白介素1α(IL1a)多克隆抗体 WB; IHC; ICC; IP.
LAA071Cp71 白介素1α(IL1a)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA071Cp22 白介素1α(IL1a)单克隆抗体 WB; IHC; ICC; IP.
LAA071Cp72 白介素1α(IL1a)单克隆抗体(生物素标记) WB; IHC; ICC.
HEA071Cp 白介素1α(IL1a)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA071Cp 白介素1α(IL1a)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA071Cp 白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSA071Cp01 白介素1α(IL1a)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA071Cp01 白介素1α(IL1a)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
The Veterinary Journal Quantitative assessment of hsp70, IL-1β and TNF-α in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathy [Pubmed: 24662024]
Int J Clin Exp Pathol Expression of S100 family proteins in neonatal rats with sepsis and its significance [PubMed: 25973048]
Tumor Biology Hypoxia-induced secretion of IL-17 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
Parasites & Vectors Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection. [pubmed:27502394]
Cell Death Dis. Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy [pubmed:28333148]
Journal of Cosmet Dermatol. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349]
Scientific Reports Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665]
Arthritis Research and Therapy Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis. [pubmed:29191221]
International Journal of Molecular Sciences Screening of circular RNAs and validation of circANKRD36 associated with inflammation in patients with type 2 diabetes mellitus [Pubmed:30066828]
Journal of Molecular Neuroscience Neuroprotective effect of curcumin against cerebral ischemia-reperfusion via mediating autophagy and inflammation [Pubmed:29243061]
Journal of Maternal-Fetal & Neonatal Medicine Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage [Pubmed:29357727]
Brazilian Dental Journal Radiographic and Immunohistochemical Evaluation of Root Canal Treatment Using Different Irrigation Systems [Pubmed: 30970054]
The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency []
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus []
Polish Journal of Veterinary Sciences Effect of postpartum administration of ketoprofen on proinflammatory cytokine concentration and their correlation with lipogenesis and ketogenesis in Holstein … [Pubmed: 31560476]
Nanomedicine The toxicity of alumina nanoparticles to immune system in mice [Pubmed: 32162999]
Theranostics Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regeneration [Pubmed: 32308770]
STEM CELLS AND DEVELOPMENT IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways [Pubmed: 33107399]
FASEB JOURNAL ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway [Pubmed: 32908567]
BIOLOGICAL TRACE ELEMENT RESEARCH Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225]
Cancer Lett Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis [34256094]
Brain Res Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells [34780748]
Animals (Basel) Inflammatory markers in uterine lavage fluids of pregnant, non-pregnant, and intrauterine device implanted mares on Days 10 and 15 post ovulation [34944269]
Animals (Basel) Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation … [Pubmed:34944269]
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