干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Interferon Gamma (IFNg)

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

  • 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA049Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测干扰素γ(IFNg),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的干扰素γ(IFNg)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 93-102 98
EDTA plasma(n=5) 78-101 93
heparin plasma(n=5) 99-105 102

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的干扰素γ(IFNg),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中干扰素γ(IFNg)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 96-103% 81-99% 94-102%
EDTA plasma(n=5) 78-96% 83-94% 88-103% 79-97%
heparin plasma(n=5) 91-99% 97-105% 80-90% 94-102%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

试剂盒内容

试剂名称 数量 试剂名称 数量
96孔板(预包被) 1 96孔板覆膜 4
标准品 2 标准品稀释液 1×20mL
检测溶液A 1×120µL 检测稀释液A 1×12mL
检测溶液B 1×120µL 检测稀释液B 1×12mL
TMB底物 1×9mL 终止液 1×6mL
浓洗涤液 (30 × concentrate) 1×20mL 说明书 1

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

ELISA Kit for Interferon Gamma (IFNg)

实验原理

将干扰素γ(IFNg)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的干扰素γ(IFNg)与连接于固相载体上的抗体结合,然后加入生物素化的干扰素γ(IFNg)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的干扰素γ(IFNg)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APA049Ra01 干扰素γ(IFNg)活性蛋白 Cell culture; Activity Assays.
EPA049Ra61 干扰素γ(IFNg)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA049Ra02 干扰素γ(IFNg)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA049Ra61 干扰素γ(IFNg)活性蛋白 Cell culture; Activity Assays.
RPA049Ra01 干扰素γ(IFNg)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA049Ra01 干扰素γ(IFNg)多克隆抗体 WB; IHC; ICC; IP.
PAA049Ra02 干扰素γ(IFNg)多克隆抗体 WB; IHC; ICC; IP.
PAA049Ra06 干扰素γ(IFNg)多克隆抗体 WB; IHC; ICC; IP.
LAA049Ra81 干扰素γ(IFNg)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA049Ra71 干扰素γ(IFNg)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA049Ra21 干扰素γ(IFNg)单克隆抗体 WB; IHC; ICC; IP.
MEA049Ra 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA049Ra 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA049Ra 干扰素γ(IFNg)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA049Ra01 干扰素γ(IFNg)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".
CGA049Ra01 干扰素γ(IFNg)cDNA PCR、蛋白表达等

参考文献

杂志 参考文献
Vaccine Bacillus Calmette-Guérin vaccination using a microneedle patch [PubMed: 21277407]
Journal of Animal and Veterinary Advances Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine [Medwell: 2010610616]
Journal of Animal and Veterinary Advances Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus [Medwell: 2010594600]
PLoS ONE Vaccination with Recombinant Mycobacterium tuberculosis PknD Attenuates Bacterial Dissemination to the Brain in Guinea Pigs [PubMed: PMC3679071]
Veterinary Research Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in?Escherichia coli?induce potent protective immune responses in guinea pigs, swine and cattle [PubMed: PMC3720265]
International Journal of Poultry Science Immune Responses to Eimeria tenella Sporozoite Protein as Vaccine to Broiler Against Coccidiosis [Pjbs: Source]
European Journal of Pharmacology Protective effects of neferine on amiodarone-induced pulmonary fibrosis in mice [Pubmed: 23792144]
Life Sci. Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model [Pubmed:25476829]
Global Journal of Pharmacology Protective Effect of Minocycline Against Bacterial Infection-Induced Sickness Behavior in Rats [Org:Source]
PLoS One. Development of CpG-Oligodeoxynucleotides for Effective Activation of Rabbit TLR9 Mediated Immune Responses [Pubmed:25269083]
Oncotarget Senescence of tumor cells induced by oxaliplatin increases the efficiency of a lipid A immunotherapy via the recruitment of neutrophils [Pubmed:25347345]
Journal of Surgical Research Exogenous carcinoembryonic antigen-related cell adhesion molecule 1 suppresses 2,4,6-trinitrobenzene sulfonic acid-induced ulcerative colitis in mice [Pubmed:25724769]
Genet Mol Res Protective effect and mechanism of probucol in the treatment of spinal cord injury [PubMed: 26214485]
Biomedical Research The toxic effect of nickel nanoparticles on oxidative stress and inflammatory markers. [Articles: ]
Mol Med Rep Roles of lipoxin A4 receptor activation and anti-interleukin-1β antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-κB pathway in airway inflammation induced by ovalbumin [PubMed: 25760938]
veterinary research forum Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ]
Immunologic Research Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE12 and is crucial for liver transplant tolerance [PubMed: 25982596]
Parasitol Res Trypanosoma cruzi infection and benznidazole therapy independently stimulate oxidative status and structural pathological remodeling of the liver tissue in mice [PubMed: 25912691]
International Journal of Immunopathology & Pharmacology Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638]
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173]
Advanced Functional Materials Nanogel‐Incorporated Physical and Chemical Hybrid Gels for Highly Effective Chemo–Protein Combination Therapy [Doi: 10.1002]
Vet Immunol Immunopathol Immune responses of pigs immunized with a recombinant porcine reproductive and respiratory syndrome virus expressing porcine GM-CSF [PubMed: 26300317]
Virol J Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4 [PubMed: 26573719]
J Interferon Cytokine Res Possible Association of IFN-γ Gene [PubMed: 26154533]
Oncotarget Rituximab-induced HMGB1 release is associated with inhibition of STAT3 activity in human diffuse large B-cell lymphoma [PubMed: 26315113]
Immunol Res Construction of recombinant Mip-FlaA dominant epitope vaccine against Legionella pneumophila and evaluation of the immunogenicity and protective immunity [PubMed: 26607265]
Asian J Pharm Clin Res INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS [journals:3790]
Life Sci. Carcinoembryonic antigen related cellular adhesion molecule 1 alleviates dextran sulfate sodium-induced ulcerative colitis in mice [Pubmed:26898127]
United States Patent Application HERPES VIRUS VACCINES AND TREATMENTS [y2016:0067330.html]
Front Pharmacol Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata [pubmed:27803670]
BMC Veterinary Research Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis [pubmed:27553600]
BMC Complementary and Alternative Medicine Reno-protective effect and mechanism study of Huang Lian Jie Du Decoction on lupus nephritis MRL/lpr mice [pubmed:27825337]
immunology letters Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila. [pubmed:28366526]
Antiviral research Evaluation of two strains of Marek's disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus. [pubmed:27908832]
Vaccine. Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J. [pubmed:28049588]
immunology letters Gemcitabine treatment enhanced the anti-tumor effect of cytokine induced killer cells by depletion of CD4+CD25bri regulatory T cells. [pubmed:27867030]
Taylor & Francis Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation [09540105.2017.1306494]
American Journal of Translational Research Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology [pubmed:27830033]
Molecular Immunology Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma [pubmed:27289030]
Basrah Journal of Veterinary Research EVALUATION OF HUMORAL AND CELLULAR IMMUNE RESPONSES TO EIMERIA TENELLA OOCYST PROTEIN AS VACCINE TO BROILER [363-379.pdf]
Journal of Physiology and Pharmacology Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury. [pubmed:28195073]
Journal of animal physiology and animal nutrition Effects of dietary mixture of garlic (Allium sativum), coriander (Coriandrum sativum) andprobiotics on immune responses and caecal counts in young laying hens. [pubmed:27678135]
Parasitology. Nonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease in T. cruzi-infected mice. [pubmed:28134069]
Vet Immunol Immunopathol.  Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus. [pubmed:28063474]
Sci Rep.  Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. [pubmed:28220870]
Sci Rep.  Assessment of the efficacy of two novel DNA vaccine formulations against highly pathogenic Porcine Reproductive and Respiratory Syndrome Virus. [pubmed:28157199]
Acta Vet Hung. Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. [pubmed:28244338]
留言咨询