High Sensitive ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)

HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78

  • 低氧诱导因子1α(HIF1a)检测试剂盒(酶联免疫吸附试验法,高敏型) 产品包装(模拟)
  • 低氧诱导因子1α(HIF1a)检测试剂盒(酶联免疫吸附试验法,高敏型) 产品包装(模拟)
  • 低氧诱导因子1α(HIF1a)检测试剂盒(酶联免疫吸附试验法,高敏型) 实验结果图
  • HEA798Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证





样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-95 90
EDTA plasma(n=5) 92-101 97
heparin plasma(n=5) 87-101 93


精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%



样本 1:2 1:4 1:8 1:16
serum(n=5) 81-91% 98-105% 78-91% 83-105%
EDTA plasma(n=5) 97-105% 98-105% 97-105% 97-104%
heparin plasma(n=5) 96-104% 88-98% 97-105% 84-102%




1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。




编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA798Hu02 低氧诱导因子1α(HIF1a)活性蛋白 Cell culture; Activity Assays.
RPA798Hu01 低氧诱导因子1α(HIF1a)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA798Hu02 低氧诱导因子1α(HIF1a)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA798Hu01 低氧诱导因子1α(HIF1a)多克隆抗体 WB; IHC; ICC; IP.
PAA798Hu02 低氧诱导因子1α(HIF1a)多克隆抗体 WB; IHC; ICC; IP.
LAA798Hu81 低氧诱导因子1α(HIF1a)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA798Hu71 低氧诱导因子1α(HIF1a)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA798Hu21 低氧诱导因子1α(HIF1a)单克隆抗体 WB; IHC; ICC; IP.
MAA798Hu22 低氧诱导因子1α(HIF1a)单克隆抗体 WB; IHC; ICC; IP.
SEA798Hu 低氧诱导因子1α(HIF1a)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA798Hu 低氧诱导因子1α(HIF1a)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA798Hu 低氧诱导因子1α(HIF1a)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA798Hu01 低氧诱导因子1α(HIF1a)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".


杂志 参考文献
Cancer Chemotherapy and Pharmacology Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells. [PubMed: 19466611]
European Journal of Pharmacology Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibition [ScienceDirect: S0014299909011352]
PLoS ONE Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor Prognosis [PubMed: PMC3781153]
International Journal of Molecular Medicine Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic rats [Pubmed: 24002353]
Canadian Journal of Physiology and Pharmacology Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemia [Pubmed: 24289078]
US8569372 B2 Method for preventing or treating a disease, disorder or condition induced by retina ischemia [US8569372]
J Surg Res. Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats. [Pubmed: 23816242]
PLoS One. HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis [Plos: Source]
Journal of Physiological Anthropology Telomeres are elongated in rats exposed to moderate altitude [Pubmed:Pmc4088304]
Molecular Medicine Reports Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein. [Pubmed:25384583]
International Journal of Pharmaceutics The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF [Pubmed:25445537]
International Journal of Molecular Medicine Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats [Pubmed:25524396]
International Journal of Developmental Neuroscience Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birth [PubMed: 25889224]
Eur Surg Res Determination of the Preferred Conditions for the Isolated Perfusion of Porcine Kidneys [PubMed: 25341590]
Sci Rep Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system [PubMed: 25905434]
Evidence-Based Complementary and Alternative Medicine Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae [pmc:PMC4794592]
Biol Trace Elem Res. Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α [Pubmed:27121973]
Раны и раневые инфекции. Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией [91]
Biological Trace Element Research Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α. [pubmed:27121973]
Experimental and Therapeutic Medicine Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis [etm:13]
Clinical Trials in Degenerative Diseases Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial [issn:2542-3975]
Annals of Clinical & Laboratory Science R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme [pubmed:28667042]
Archives of Gynecology and Obstetrics Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia? [10.1007/s00404-017-4461-2]
EXPERIMENTAL AND THERAPEUTIC MEDICINE Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee [pubmed:28123469]
Medical Journal Armed Forces India Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003]