组胺(HA)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay)

Histamin

（注：单次混测多因子不超过8个指标 ）

  • 组胺(HA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 组胺(HA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测组胺(HA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的组胺(HA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-95 89
EDTA plasma(n=5) 94-101 97
heparin plasma(n=5) 90-97 94

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的组胺(HA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中组胺(HA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 81-104% 93-102% 82-95% 81-103%
EDTA plasma(n=5) 80-101% 97-105% 89-97% 79-94%
heparin plasma(n=5) 79-89% 81-101% 80-105% 85-99%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将组胺(HA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的组胺(HA)等多因子检测试剂盒(流式荧光发光法)和未标记的组胺(HA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的组胺(HA)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Pan-species (General，通用) 应用(仅供研究使用，不用于临床诊断！)
CPA927Ge41 组胺(HA)人血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA927Ge11 组胺(HA)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA927Ge21 组胺(HA)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA927Ge01 组胺(HA)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA927Ge71 组胺(HA)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA927Ge81 组胺(HA)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA927Ge21 组胺(HA)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA927Ge 组胺(HA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA927Ge 组胺(HA)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA927Ge 组胺(HA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA927Ge11 组胺(HA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It(ELISA Kit) Yourself"

参考文献

杂志 参考文献
Allergy & Clinical Immunology PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in mice [Pubmed: 21981059]
Asian Pacific Journal of Allergy and Immunology Comparison of sensitization between β-lactoglobulin and its hydrolysates [Digital: 875]
J Chromatogr A Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection [PubMed: 26372445]
Neurogastroenterology & Motility Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea [Pubmed:26940641]
Italian Journal of Animal Science Induction of immune responses and allergic reactions in piglets by injecting glycinin [doi:1828051X.2016.1144488]
Int J Clin Exp Pathol Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism [files:ijcep0019305.pdf]
PLoS One. Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbumin [pmc:PMC4734762]
Int Immunopharmacol. Evaluation of anaphylactoid constituents in vitro and in vivo. [pubmed:27984711]
Journal of Cosmet Dermatol. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605]
Royal Society Open Science Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats []
International Journal of Pharmaceutical Sciences and Research MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL []
Allergy Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial [Pubmed: 32030780]
MOLECULAR IMMUNOLOGY Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806]
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767]
Nature Communications A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy [Pubmed: 32332752]
CCR3‑shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells [Pubmed: 32742345]
ANALYTICA CHIMICA ACTA Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of … [Pubmed: 32892931]
Annals of Tropical Medicine & Public Health Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq []
pharmacol res& perspect Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs [34014033]
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