Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Procollagen II C-Terminal Propeptide (PIICP) ,etc. by FLIA (Flow Luminescence Immunoassay)

P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

（注：单次混测多因子不超过8个指标 ）

  • Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 83-97 93
EDTA plasma(n=5) 81-91 88
heparin plasma(n=5) 90-101 93
sodium citrate plasma(n=5) 79-95 86

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 82-97% 89-99% 97-105%
EDTA plasma(n=5) 84-101% 90-104% 78-98% 89-99%
heparin plasma(n=5) 83-91% 92-103% 81-105% 91-105%
sodium citrate plasma(n=5) 84-103% 98-105% 92-105% 84-104%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法)和未标记的Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA964Mu01 Ⅱ型前胶原羧基端原肽(PIICP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA964Mu21 Ⅱ型前胶原羧基端原肽(PIICP)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA964Mu08 Ⅱ型前胶原羧基端原肽(PIICP)多克隆抗体 WB; IHC; ICC; IP.
PAA964Mu01 Ⅱ型前胶原羧基端原肽(PIICP)多克隆抗体 WB; IHC; ICC; IP.
CEA964Mu Ⅱ型前胶原羧基端原肽(PIICP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA964Mu Ⅱ型前胶原羧基端原肽(PIICP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA964Mu11 Ⅱ型前胶原羧基端原肽(PIICP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Experimental and Therapeutic Medicine Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: A … [10.3892]
Functional Foods in Health & Disease Evaluation of the effect of administering N-acetyl-glucosamine-containing green tea supplement on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: a randomized double-blind placebo-controlled clinical study [309]
EXPERIMENTAL AND THERAPEUTIC MEDICINE Effect of N-acetylglucosamine administration on cartilage metabolism and safety in healthy subjects without symptoms of arthritis: A case report [pubmed:28413518]
Experimental and Therapeutic Medicine Evaluation of the effect of salmon nasal proteoglycan on biomarkers for cartilage metabolism in individuals with knee joint discomfort: A randomized double‑blind placebo‑controlled clinical study [etm:14]
Experimental and Therapeutic Medicine Evaluation of the efficacy of Ajuga decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double‑blind, placebo‑controlled study [10.3892/etm.2017.5064]
EXPERIMENTAL AND THERAPEUTIC MEDICINE Evaluation of the chondroprotective action of N‑acetylglucosamine in a rat experimental osteoarthritis [pubmed:28912864]
Functional Foods In Health And Disease Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals: a randomized double-blind placebo-controlled clinical study [view/366]
Spine No effects of hyperosmolar culture medium on tissue regeneration by human degenerated nucleus pulposus cells despite upregulation extracellular matrix genes [fulltext:2018/03010]
Advances in Clinical and Experimental Medicine Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with … [68362.pdf]
Functional Food Research サケ鼻軟骨由来プロテオグリカン摂取による関節保護効果 []
Spine No Effects of Hyperosmolar Culture Medium on Tissue Regeneration by Human Degenerated [Pubmed: 25856264]
Applied Physiology Nutrition and Metabolism Effectiveness of collagen supplementation on pain scores in healthy individuals with self-reported knee pain; A randomized controlled trial [Pubmed: 31990581]
International Journal of Molecular Sciences Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration [Pubmed: 32295299]
Biomolecules Concerted Actions by PIICP, CTXII, and TNF-¦Á in Patients with Juvenile Idiopathic Arthritis [33924892]
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