血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Vascular Cell Adhesion Molecule 1 (VCAM1) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD106; INCAM-100; L1CAM

（注：单次混测多因子不超过8个指标 ）

  • 血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 91-102 97
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 92-105 101
sodium citrate plasma(n=5) 85-99 88

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-102% 87-103% 81-102% 78-99%
EDTA plasma(n=5) 81-94% 98-105% 91-99% 97-104%
heparin plasma(n=5) 90-104% 90-97% 89-102% 81-102%
sodium citrate plasma(n=5) 80-97% 94-103% 84-96% 92-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Oryctolagus cuniculus (Rabbit，兔) 应用(仅供研究使用，不用于临床诊断！)
RPA547Rb02 血管细胞粘附分子1(VCAM1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA547Rb01 血管细胞粘附分子1(VCAM1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA547Rb51 血管细胞粘附分子1(VCAM1)多克隆抗体 WB
PAA547Rb52 血管细胞粘附分子1(VCAM1)多克隆抗体 WB; IHC; ICC; IP.
LAA547Rb71 血管细胞粘附分子1(VCAM1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA547Rb21 血管细胞粘附分子1(VCAM1)单克隆抗体 WB; IHC; ICC; IP.
MAA547Rb22 血管细胞粘附分子1(VCAM1)单克隆抗体 WB; IHC; ICC; IP.
LAA547Rb72 血管细胞粘附分子1(VCAM1)单克隆抗体(生物素标记) WB; IHC; ICC.
FAA547Rb52 抗血管细胞粘附分子1(VCAM1)单克隆抗体(别藻蓝蛋白标记) FCM
SEA547Rb 血管细胞粘附分子1(VCAM1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA547Rb 血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
PSA547Rb01 血管细胞粘附分子1(VCAM1)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA547Rb01 血管细胞粘附分子1(VCAM1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Amran et al. Lipids in Health and Disease Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. [BioMed: 1476511X]
Vascular Pharmacology Long-term ethanol consumption initiates atherosclerosis in rat aorta through inflammatory stress and endothelial dysfunction [ScienceDirect: S1537189112000705]
Canadian Journal of Physiology and Pharmacology Resveratrol abrogates adhesion molecules and protects against TNBS-induced ulcerative colitis in rats. [PubMed: 22029500]
British Journal of Medical and Health Sciences EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS [Researchgate: Source]
Endocrine Metabolic & Immune Disordors Drug Targets. Active immunization against tumor necrosis factor-alpha decreases proinflammatory cytokines, oxidative stress mediators and adhesion molecules risk factors in streptozotocin-induced diabetic rats. [Pubmed: 23859636]
Atherosclerosis Increased monocyte adhesion by endothelial expression of VCAM-1 missense variation in vitro [Pubmed: 24075742]
Atherosclerosis Activation of receptor for advanced glycation end products contributes to aortic remodeling and endothelial dysfunction in sinoaortic denervated rats. [Pubmed: 23880178]
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192]
Arch Dis Child PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns [Bmj:Source]
Arch Dis Child? PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome [Bmj:Source]
Arch Dis Child PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography [Bmj:Source]
Naunyn-Schmiedeberg&#039;s Archives of Pharmacology Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits [Pubmed:25172523]
Nutr Res. Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits [Pubmed:25282128]
Alexandria Journal of Medicine Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats [Sciencedirect:S2090506814000268]
Canadian Journal of Physiology and Pharmacology Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism [Doi: Abs]
Hum Exp Toxicol Effect of agmatine on experimental vascular endothelial dysfunction [PubMed: 26424770]
International Journal of Food Sciences and Nutrition Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products [PubMed: 26401576]
J Trace Elem Med Biol Comparative angioprotective effects of magnesium compounds [PubMed: 25127069]
J Mol Cell Cardiol Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation [PubMed: 26231083]
Endocrine Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways [Pubmed:26758998]
Mol Neurobiol A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats [Pubmed:26867655]
Pakistan journal of pharmaceutical sciences Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. [pubmed:29084680]
Acta Biochim Biophys Sin Therapeutic potential of mesenchymal stem cells in acute kidney injury is affected by administration timing [pubmed:28338909]
Experimental and Therapeutic Medicine Epigallocatechin‑3‑gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression [pubmed:28962112]
Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422]
Evidence-Based Complementary and Alternative Medicine BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway [journals:1758383]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
International Journal of Clinical and Experimental Pathology Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study [ISSN:1936-2625/IJCEP0055881]
ResearchGate Significance of Soluble Endoglin as a Possible Marker of Cardiovascular Disease in Type 2 Diabetic Patients [315113405]
Regenerative Biomaterials The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic [10.1093/rb/rbx028]
Anesthesiology Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … [Pubmed:28968277]
PLoS Neglected Tropical Diseases Characterization of the rhesus macaque (Macaca mulatta) scrub typhus model: Susceptibility to intradermal challenge with the human pathogen Orientia … [Pubmed:29522521]
Life Sciences Nephroprotective effect of saxagliptin against gentamicin-induced nephrotoxicity, emphasis on anti-oxidant, anti-inflammatory and anti-apoptic effects [Pubmed:30012474]
Evidence-based Complementary and Alternative Medicine Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling … [Pubmed:29619063]
Nutrition & Metabolism L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835]
Cytokine Intercellular adhesion molecule-1 (ICAM-1) associates with 24-hour ambulatory blood pressure variability in type 2 diabetes and controls [Pubmed: 30716657]
Anticancer Research Effect of Radiation on the Expression of CVD-related miRNAs, Inflammation and Endothelial Dysfunction of HUVECs [Pubmed: 30711956]
European Journal of Pharmacology The cordycepin derivative IMM-H007 improves endothelial dysfunction by suppressing vascular inflammation and promoting AMPK-dependent eNOS activation in … [Pubmed: 30826323]
international journal of molecular sciences Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells [Pubmed: 31671764]
Archives of Insect Biochemistry and Physiology Vanadyl sulphate ameliorates biomarkers of endothelial injury and coagulation and thrombosis in a rat model of hyperglycaemia [Pubmed: 31774317]
BMC Endocrine Disorders The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children [Pubmed: 31771561]
Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827]
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE Endothelial epithelial sodium channel involves in high-fat diet-induced atherosclerosis in low-density lipoprotein receptor-deficient mice [Pubmed: 33065235]
Antioxidants (Basel) Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model [33562069]
Sci. Pharm Dehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism []
Journal of the South African Veterinary Association Circulating markers of endothelial activation in canine parvoviral enteritis []
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