髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本)

Mini Samples ELISA Kit for Myeloperoxidase (MPO)

  • 髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本) 实验结果图
  • MEA601Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 88-96 92
EDTA plasma(n=5) 79-102 83
heparin plasma(n=5) 79-104 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 92-101% 80-102% 86-101%
EDTA plasma(n=5) 86-94% 89-102% 96-105% 88-96%
heparin plasma(n=5) 79-93% 89-98% 80-99% 93-104%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)25µL,37°C温育1小时;
3. 吸弃,加检测溶液A25µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B25µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物25µL,37°C孵育10-20分钟;
8. 加终止液20µL,立即450nm读数。

实验原理

将髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合,然后加入生物素化的髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA601Ra01 髓过氧化物酶(MPO)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA601Ra02 髓过氧化物酶(MPO)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA601Ra01 髓过氧化物酶(MPO)多克隆抗体 WB; IHC; ICC; IP.
MAA601Ra21 髓过氧化物酶(MPO)单克隆抗体 WB; IHC; ICC; IP.
MEA601Ra 髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA601Ra 髓过氧化物酶(MPO)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA601Ra 髓过氧化物酶(MPO)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
International Journal of Medical Sciences Ozone Therapy and Hyperbaric Oxygen Treatment in Lung Injury in Septic Rats [PubMed: 21234269]
Respiration Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice [Karger: 000319151]
Inflammation Preventive Effects of Valnemulin on Lipopolysaccharide-Induced Acute Lung Injury in Mice [SpringerLink: 741437t67767777x]
Advanced Materials Research A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-Chitosan [Scientific:1207]
9 T cells that target carcinoembryonic antigen eradicate orthotopic pancreatic carcinomas without inducing autoimmune colitis in mice [PubMed: 22750462]
European Surgical Research Rosuvastatin Reloading before Cardiac Surgery with Cardiopulmonary Bypass [PubMed: 23406839]
Toxicology and Industrial Health Protective effects of Ankaferd blood stopper on aspirin-induced oxidative mucosal damage in a rat model of gastric injury. [PubMed: 23114375]
Experimental Lung Research Protective effect of pravastatin on lipopolysaccharide-induced acute lung injury during neutropenia recovery in mice [Pubmed: 23368428]
International Immunopharmacology Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis [Pubmed: 24126114]
Human & experimental toxicology Neuroprotective effects of α-ketoglutarate and ethyl pyruvate against motor dysfunction and oxidative changes caused by repeated 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine exposure in mice [Pubmed: 23821591]
International Immunopharmacology Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits [ScienceDirect: S1567576913004153]
PLOS ONE Novel CoQ10 Antidiabetic Mechanisms Underlie Its Positive Effect: Modulation of Insulin and Adiponectine Receptors, Tyrosine Kinase, PI3K, Glucose Transporters, sRAGE and Visfatin in Insulin Resistant/Diabetic Rats [Pubmed:24586567]
Acta Anaesthesiol Scand. Heparin-binding protein (HBP/CAP37) - a link to endothelin-1 in endotoxemia-induced pulmonary oedema? [Pubmed:24611481]
Journal of Environmental Pathology, Toxicology and Oncology Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways [Pubmed:24941292]
European Journal of Pharmacology Neuroprotective effects of rosuvastatin against traumatic spinal cord injury in rats [Pubmed:25084223]
Iran J Pharm Res. Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical Trial [Pubmed:25237354]
Chem Biol Interact.? Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activities [Pubmed:25289771]
Journal of Surgical Research Melatonin attenuates intestinal ischemia–reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2 [Pubmed:25491174]
Neurochemistry International Sulfiredoxin-1 protects primary cultured astrocytes from ischemia-induced damage [Pubmed:25620665]
Ann N Y Acad Sci.? Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis [Pubmed:5243883]
J Crohns The Syk Inhibitor Fostamatinib Decreases the Severity of Colonic Mucosal Damage in a Rodent Model of Colitis [PubMed: 26116555]
Monoclon Antib Immunodiagn Immunother Preparation of Monoclonal Antibodies and a Simple Myeloperoxidase-Immunosorbent Assay for Detecting Human Myeloperoxidase [Pubmed:26978734]
Clinics and Research in Hepatology and Gastroenterology The efficacy of tyrosine kinase inhibitor dasatinib on colonic mucosal damage in murine model of colitis [Pubmed:26823039]
Asian J Pharm Clin Res THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATS [journals:10835]
Hepatology. Toll-like receptor 5 signaling restrains T-cell/natural killer T-cell activation and protects against concanavalin A-induced hepatic injury. [pubmed:28273362]
International Journal of Sports Physiology and Performance  Exploring the Potential of cfDNA Measurements After an Exhaustive Cycle Ergometer Test as a Marker for Performance Related Parameters [pubmed:27617485]
Journal of Pharmacy and Pharmaceutical Sciences Ellagic Acid Protects Against LPS-Induced Acute Lung Injury Through Inhibition of Nuclear Factor Kappa B, Proinflammatory Cytokines and Enhancement of Interleukin-10 [c30adb97b06eb4f27063208df7acb7311368.pdf]
Future Microbiology Transplantation of a bacterial consortium ameliorates trinitrobenzenesulfonic acid-induced colitisand intestinal dysbiosis in rats. [pubmed:27415182]
The Annals of Thoracic Surgery Sevoflurane Attenuates Ischemia-Reperfusion Injury in a Rat Lung Transplantation Model. [pubmed:28190546]
Biological Rhythm Research Fluctuation in the number, type and activity of blood neutrophils in cows exhibiting successful and unsuccessful completion of gestation cycle [09291016.2017.1311984]
Journal of Cancer Research and Treatment Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines [jcrt:5]
Identification of Tengfu Jiangya Tablet Target Biomarkers [journals:ecam]
Hepatology. Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis. [pubmed:27775820]
LAB ON A CHIP Enzyme catalysis-electrophoresis titration for multiplex enzymatic assay via moving reactionboundary chip. [pubmed:27464600]
Indian Journal of Animal Research Effect of Poly-herbal mixture and butyric acid feeding on immune parameters of postpartum Murrah buffaloes [publication:299575009]
The American Journal of Cardiology Frequency of Cholesterol Crystals in Culprit Coronary Artery Aspirate During Acute Myocardial Infarction and Their Relation to Inflammation and Myocardial Injury [pubmed:28867129]
Evidence-Based Complementary and Alternative Medicine Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique [pubmed:28408942]
Lasers in Medical Science Effect of photobiomodulation therapy on reducingthe chemo-induced oral mucositis severity and on salivary levels of CXCL8/interleuki8, nitrite, and myeloperoxidase in patients undergoing hematopoietic stem cell transplantation: a randomized clinical trial [article:10.1007]
Experiental And Therapeutic Medicine Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription [pubmed:28672924]
Frontiers in Immunology Upregulation of Intestinal Barrier Function in Mice with DSS-Induced Colitis by a Defined Bacterial Consortium Is Associated with Expansion of IL-17A Producing Gamma Delta T Cells [10.3389:fimmu.2017.00824]
Journal of Pediatric Surgery Does penile tourniquet application alter bacterial adhesion to rat urethral cells: an in vitro study [pubmed:28693848]
Food and Agricultural Immunology Ellagic acid protects against LPS-induced acute lung injury through inhibition of nuclear factor kappa B, proinflammatory cytokines and enhancement of interleukin-10 [10.1080/09540105.2017.1339670]
Oxidative Medicine and Cellular Longevity Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils [pubmed:29081886]
Advanced Science Letters Serum Myeloperoxidase in Stable Coronary Artery Disease [10.1166/asl.2017.8512]
Tropical Journal of Pharmaceutical Research Hepatoprotective effect of taurine and coenzyme Q10 and their combination against acrylamide-induced oxidative stress in rats [10.4314/tjpr.v16i8.14]
deposit once Role of NOD2 and S100A8/S100A9 in the pathogenesis of Coxsackievirus B3-induced myocarditis [10.14279/depositonce-5934]
Archives of Medical Research Effects of Sulphasalazine in Cerebral Ischemia Reperfusion Injury in Rat. [pubmed:28923326]
Frontiers in Immunology The Plasma Kallikrein–Kininogen Pathway is critical in the Pathogenesis of colitis in Mice [Pubmed:29467753]
Frontiers in Pharmacology Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal … [Pubmed:29375374]
Biomedicine & Pharmacotherapy Evaluation of the anti-hypertensive effect of Tengfu Jiangya tablet by combination of UPLC-Q-exactive-MS-based metabolomics and iTRAQ-based proteomics … [Pubmed:29453042]
Molecular Vision Resolvin D1 promotes corneal epithelial wound healing and restoration of mechanical sensation in diabetic mice [Pubmed:29643724]
Theriogenology Effect of tropical thermal stress on peri-implantation immune responses in cows [Pubmed:29625402]
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781]
Oncology Letters Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of … [Pubmed:29928398]
Journal of Veterinary Science Associations of periparturient plasma biochemical parameters, endometrial leukocyte esterase and myeloperoxidase, and bacterial detection with clinical and … [Pubmed:29332864]
Life Sciences Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent manner [Pubmed: 30195035]
International Journal of Clinical and Experimental Medicine Effect of β-glucan on acetic acid-induced colitis in rats []
BioMed Research International Chlorogenic Acid Attenuates Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice through MAPK/ERK/JNK Pathway []
Beneficial Microbes Bacillus licheniformis Zhengchangsheng® attenuates DSS-induced colitis and modulates the gut microbiota in mice []
General Medicine: Open Access Do Late Phase Hyperbaric and Normobaric Oxygen Therapies have Effect on Liver Damage?: An Experimental Sepsis Model []
journal of surgical research Posttreatment With LYRM03 Protects Rats From Acute Lung Inflammation Induced by Lipopolysaccharide via Suppressing the NF-κB/MyD88/TLR4 Axis [Pubmed: 31255931]
Toxicol Ind Health Efficacy assessment of co-treated alpha-ketoglutarate and N-acetyl cysteine against the subchronic toxicity of cyanide in rats [Pubmed: 31244408]
Journal of Pediatric Surgery The effects of hydrogen-rich saline solution on intestinal anastomosis performed after intestinal ischemia reperfusion injury []
留言咨询