转酮醇酶(TKT)多克隆抗体
Polyclonal Antibody to Transketolase (TKT)
TK; Wernicke-Korsakoff Syndrome
- 编号PAH019Ra01
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPH019Ra01-转酮醇酶(TKT)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度500µg/mL
- 且适物种Homo sapiens (Human，人)
- 应用WB,IHC
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 684 ¥ 1596 ¥ 2280 ¥ 5700 ¥ 22800
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Rat Colon Tissue; Primary Ab: 10µg/ml Rabbit Anti-Rat TKT Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Liver Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat TKT Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Spinal cord Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat TKT Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Colon Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat TKT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat TKT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Spinal cord Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat TKT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant TKT, Rat.
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Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Rat TKT Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: TKT knockout Hela cell lysate;
Predicted MW: 68kd
Observed MW: 70kd
Primary Ab: 1µg/ml Rabbit Anti-Rat TKT Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对TKT的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别TKT。
用法
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPH019Ra01 | 转酮醇酶(TKT)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH019Ra01 | 转酮醇酶(TKT)多克隆抗体 | WB,IHC |
| MAH019Ra21 | 转酮醇酶(TKT)单克隆抗体 | WB; IHC; ICC; IP. |
| SEH019Ra | 转酮醇酶(TKT)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMH019Ra | 转酮醇酶(TKT)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| JOURNAL OF PROTEOME RESEARCH | Discovery of Novel Protein Biomarkers in Urine for Diagnosis of Urothelial Cancer Using iTRAQ Proteomics [33780252] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins [] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212] |
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