绿色荧光蛋白(GFP)重组抗体

Recombinant Antibody to Green Fluorescent Protein (GFP)

  • 绿色荧光蛋白(GFP)重组抗体 产品包装(模拟)
  • 绿色荧光蛋白(GFP)重组抗体 产品包装(模拟)
  • 绿色荧光蛋白(GFP)重组抗体 Briefly, biotin labelled GFP Nano-antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to GFP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of GFP and GFP Nano-antibody was shown in Figure 1, and the ED50 for this effect is 0.0187 ug/ml.
  • 绿色荧光蛋白(GFP)重组抗体 GFP was immunoprecipitated 293F whole cell extract (Expressing GFP) with 2 µg of RAD025Ge71 and 20 µl of Nickel pillar magnetic beads ( ). 
    Western blot was perfomed on the immunoprecipitate using 0.05µg/ml MAD025Ge21 and 0.2µg/mL SAA544Mu19.
    Lane1 (input): 293F whole cell lysate.
    Lane2 ( ): RAD025Ge71 in 293F whole cell lysate.
    Lane3 (-): No antibody was added to the 293F whole cell lysate.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对GFP的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GFP。

用法

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
EPD025Ge51 绿色荧光蛋白(GFP)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPD025Ge01 绿色荧光蛋白(GFP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPD025Ge02 绿色荧光蛋白(GFP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAD025Ge07 绿色荧光蛋白(GFP)多克隆抗体 WB; IHC; ICC; IP.
PAD025Ge02 绿色荧光蛋白(GFP)多克隆抗体 WB; IHC; ICC; IP.
PAD025Ge51 绿色荧光蛋白(GFP)多克隆抗体 WB; IHC; ICC; IP.
MAD025Ge21 绿色荧光蛋白(GFP)单克隆抗体 WB; IHC; ICC; IP.
MAD025Ge22 绿色荧光蛋白(GFP)单克隆抗体 WB; IHC; ICC; IP.
RAD025Ge71 绿色荧光蛋白(GFP)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAD025Ge51 绿色荧光蛋白(GFP)单克隆抗体 WB; IHC; ICC; IP.
SED025Ge 绿色荧光蛋白(GFP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
AED025Ge 抗绿色荧光蛋白抗体(Anti-GFP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
LMD025Ge 绿色荧光蛋白(GFP)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSD025Ge01 绿色荧光蛋白(GFP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Explore the activation efficiency of different ligand carriers on synNotch-based contact-dependent activation system []
FASEB J CircPrkcsh, a circular RNA, contributes to the polarization of microglia towards the M1 phenotype induced by spinal cord injury and acts via the JNK/p38 MAPK … [34751973]
Microbiol Spectr Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells [Pubmed:35604158]
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