Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围)

Wide-range ELISA Kit for Salusin Beta (SALb)

  • Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围) 产品包装(模拟)
  • Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围) 产品包装(模拟)
  • Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围) 实验结果图
  • WEC026Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 94-101 97
EDTA plasma(n=5) 88-95 92
heparin plasma(n=5) 95-103 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 98-105% 97-105% 82-103%
EDTA plasma(n=5) 79-97% 91-103% 92-101% 94-101%
heparin plasma(n=5) 82-95% 90-104% 95-104% 85-94%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
CPC026Hu21 Salusin肽β(SALb)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAC026Hu08 Salusin肽β(SALb)多克隆抗体 WB; IHC; ICC; IP.
PAC026Hu01 Salusin肽β(SALb)多克隆抗体 WB; IHC; ICC; IP.
WEC026Hu Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
CEC026Hu Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSC026Hu11 Salusin肽β(SALb)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Journal of Maternal-Fetal and Neonatal Medicine Serum levels of apelin, salusin-alpha, and salusin-beta in normal pregnancy and preeclampsia [Informa: 14767058.2012.660221]
Peptides The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass [PubMed: 22884920]
Department of Medical Biochemistry and Clinical Biochemistry Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows [ScienceDirect: S0196978113000533]
European Journal of Obstetrics & Gynecology and Reproductive Biology Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. [PubMed: 23178004]
Biotech Histochem. Serum concentration and kidney expression of salusin-α and salusin-β in rats with metabolic syndrome induced by fructose. [PubMed: 23244237]
Gynecological Endocrinology, Salusins, newly identified regulators of hemodynamics and mitogenesis, increase in polycystic ovarian syndrome. [PubMed: 22827297]
Peptides Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus [ScienceDirect: S0196978113003100]
Biotechnic &amp; Histochemistry Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome [Com:Source]
Med Sci Monit. Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome [Pubmed:Pmc4136931]
Acta Physiologica Superoxide anions involved in sympathoexcitation and pressor effects of salusin-β in paraventricular nucleus in hypertensive rats [Pubmed:24304512]
Pediatr Nephrol. Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? [Pubmed:25245503]
European Journal of Dermatology Serum salusin-α and salusin-β levels in patients with psoriasis [PubMed: 26055619]
J Investig Med Serum Salusin-β Levels Are Associated With the Presence and Severity of Coronary Artery Disease [PubMed: 25730454]
Genet Test Mol Biomarkers.  Serum Salusin-β Levels Are Correlated with Slow Coronary Flow. [Pubmed:27227272]
Sci Rep.  Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. [pubmed:28230187]
Cell Death Dis. Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy [pubmed:28333148]
Oxidative Medicine and Cellular Longevity Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma [10.1155:2017]
International Journal Of Cardiology Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus [Pubmed: 30581101]
Multiple Sclerosis and Related Disorders Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis [Pubmed: 30738876]
留言咨询