髓鞘碱性蛋白(MBP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Myelin Basic Protein (MBP)

Myelin A1 protein; Myelin membrane encephalitogenic protein

特异性

试剂盒中的抗体对髓鞘碱性蛋白(MBP)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
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UMAA539Hu21 髓鞘碱性蛋白(MBP)单克隆抗体 WB; IHC; ICC; IP.
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参考文献

杂志 参考文献
PLOS ONE CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source]
Journal of Occupational Health Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain [Pubmed: 23183024]
Pak J Med Sci Myelin basic protein and ischemia modified albumin levels in acute ischemic stroke cases [PubMed: 26648996]
Experimental and Toxicologic Pathology Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus) [Pubmed:27165445]
Acta Angiologica Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy [AA.2016.0008]
Stem Cells. Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. [pubmed:27573615]
Environmental Science and Pollution Research Glial markers and emotional memory in rats following acute cerebral radiofrequency exposures. [pubmed:27696165]
Acta Angiol Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy [AA.2016.0008]
PLoS Neglected Tropical Diseases Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis [pubmed:28489854]
Pesticide Biochemistry and Physiology Acrolein-mediated neurotoxicity in growing Wistar male rats [Pubmed:30033014]
International Journal of Livestock Research Association and Risk of Canine Distemper with Respect to Age, Sex and Breed of Dogs Suffering from Demyelinating Neuropathies [10.5455:ijlr.20170811080455]
Microchimica Acta Biosensors and nanobiosensors for rapid detection of autoimmune diseases: a review [Pubmed: 31760523]
The role of light desynchronosis in the development of stress-induced aging []
Adv Med Sci Association of eosinophil-mediated inflammatory biomarkers with the presence of the Schatzki ring [34091432]
biomolecules Searching for Noninvasive Predictors of the Diagnosis and Monitoring of Eosinophilic Esophagitis¡ªThe Importance of Biomarkers of the Inflammatory Reaction?¡­ []
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