精氨酸酶(ARG)活性蛋白

Active Arginase (ARG)

ARG1; Arginase I; Liver Arginase

  • 精氨酸酶(ARG)活性蛋白 产品包装(模拟)
  • 精氨酸酶(ARG)活性蛋白 产品包装(模拟)
  • 精氨酸酶(ARG)活性蛋白 Gene sequencing
  • APB120Hu01.jpg Figure. SDS-PAGE
  • 精氨酸酶(ARG)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Arginase (ARG) is a kind of inducible manganese (Mn2 )-containing enzymes that catalyse the final step of the urea cycle in the liver, converting l -arginine into l -ornithine and urea.Therefore, we can measure its bioactivity by enzyme activity assay. Recombinant human Arg was diluted to different concentrations in deionized water.Loading 40 µL of the rhARG1 and 10ul Substrate mixture(100 mM Arginine, 500 mM Glycine, 1.25 mM MnCl2, pH 10.5 ) into the plate. Incubated at 37 °C for 2 hours and stop the reaction with 200 µL stop solution of the 2 mM oPA, 2 mM NED ,50 mM Boric Acid, 1 M Sulfuric Acid, 0.03% Brij-35 (w/v) .Cover the plate and incubate at room temperature for 20 minutes and read at 520 nm (absorbance) in endpoint mode. The specific activity of recombinant human ARG is >24400pmol/min/ug.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPB120Hu01 精氨酸酶(ARG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB120Hu01 精氨酸酶(ARG)活性蛋白 Cell culture; Activity Assays.
PAB120Hu01 精氨酸酶(ARG)多克隆抗体 WB; IHC; ICC; IP.
LAB120Hu71 精氨酸酶(ARG)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB120Hu81 精氨酸酶(ARG)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB120Hu22 精氨酸酶(ARG)单克隆抗体 WB; IHC; ICC; IP.
MAB120Hu23 精氨酸酶(ARG)单克隆抗体 WB; IHC; ICC/IF.
MAB120Hu24 精氨酸酶(ARG)单克隆抗体 WB; IHC; ICC; IP.
MAB120Hu21 精氨酸酶(ARG)单克隆抗体 WB; IHC
LAB120Hu72 精氨酸酶(ARG)单克隆抗体(生物素标记) WB; IHC; ICC.
SEB120Hu 精氨酸酶(ARG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB120Hu 精氨酸酶(ARG)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB120Hu01 精氨酸酶(ARG)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
PLoS One Phenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat Livers [Pubmed: 24204595]
J Cereb Blood Flow Metab Arginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of stroke [PubMed: 25966956]
Cancer Medicine Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease [Pubmed:27109254]
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496]
Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells []
Commun Biol Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells [34893694]
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