B-淋巴细胞趋化因子(BLC)活性蛋白
Active B-Lymphocyte Chemoattractant (BLC)
CXCL13; SCYB13; BCA1; BLR1L; ANGIE; ANGIE2; Chemokine(C-X-C-Motif)ligand 13; Small Inducible Cytokine B Subfamily(Cys-X-Cys Motif)Member 13
- 编号APB601Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点10.4
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1267 ¥ 3168 ¥ 6336 ¥ 19008 ¥ 47520
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活性实验

Lymphocyte Chemoattractant 1 (BLC1) , also known as CXC chemokine ligand 13 (CXCL13) is a member of the CC chemokine family andis constitutively expressed in secondary lymphoid organs. . BLC1 functions by attracting B lymphocytes to sites of inflammation, thus facilitating the immune response against pathogens. The protein is involved in various physiological processes, including B cell migration, proliferation, and differentiation. Additionally, BLC1 has been associated with several diseases, including autoimmune disorders and certain types of cancer.Chemokine C-X-C-Motif Receptor 5 (CXCR5) is the cognate receptor for BLC1,, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant humant BLC1 and recombinant human CXCR5. Briefly,BLC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CXCR5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-BLC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant humant BLC1 and recombinant human CXCR5 was shown in Figure 1, the EC50 for this effect is 0.62ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
APB601Hu01 | B-淋巴细胞趋化因子(BLC)活性蛋白 | Cell culture; Activity Assays. |
RPB601Hu01 | B-淋巴细胞趋化因子(BLC)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB601Hu01 | B-淋巴细胞趋化因子(BLC)多克隆抗体 | WB,IHC |
MAB601Hu22 | B-淋巴细胞趋化因子(BLC)单克隆抗体 | WB; IHC; ICC; IP. |
SEB601Hu | B-淋巴细胞趋化因子(BLC)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB601Hu | B-淋巴细胞趋化因子(BLC)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB601Hu | B-淋巴细胞趋化因子(BLC)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | Possible Anti-inflammatory Effects of Mesenchymal Stem Cells Transplantation via Changes in CXCL8 Levels in Patients with Refractory Rheumatoid Arthritis [] |