B-淋巴细胞趋化因子(BLC)活性蛋白

Active B-Lymphocyte Chemoattractant (BLC)

CXCL13; SCYB13; BCA1; BLR1L; ANGIE; ANGIE2; Chemokine(C-X-C-Motif)ligand 13; Small Inducible Cytokine B Subfamily(Cys-X-Cys Motif)Member 13

  • B-淋巴细胞趋化因子(BLC)活性蛋白 产品包装(模拟)
  • B-淋巴细胞趋化因子(BLC)活性蛋白 产品包装(模拟)
  • APB601Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Lymphocyte Chemoattractant 1 (BLC1) , also known as CXC chemokine ligand 13 (CXCL13) is a member of the CC chemokine family andis constitutively expressed in secondary lymphoid organs. . BLC1 functions by attracting B lymphocytes to sites of inflammation, thus facilitating the immune response against pathogens. The protein is involved in various physiological processes, including B cell migration, proliferation, and differentiation. Additionally, BLC1 has been associated with several diseases, including autoimmune disorders and certain types of cancer.Chemokine C-X-C-Motif Receptor 5 (CXCR5) is the cognate receptor for BLC1,, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant humant BLC1 and recombinant human CXCR5. Briefly,BLC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CXCR5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-BLC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant humant BLC1 and recombinant human CXCR5 was shown in Figure 1, the EC50 for this effect is 0.62ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB601Hu01 B-淋巴细胞趋化因子(BLC)活性蛋白 Cell culture; Activity Assays.
RPB601Hu01 B-淋巴细胞趋化因子(BLC)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB601Hu01 B-淋巴细胞趋化因子(BLC)多克隆抗体 WB,IHC
MAB601Hu22 B-淋巴细胞趋化因子(BLC)单克隆抗体 WB; IHC; ICC; IP.
SEB601Hu B-淋巴细胞趋化因子(BLC)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB601Hu B-淋巴细胞趋化因子(BLC)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB601Hu B-淋巴细胞趋化因子(BLC)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Possible Anti-inflammatory Effects of Mesenchymal Stem Cells Transplantation via Changes in CXCL8 Levels in Patients with Refractory Rheumatoid Arthritis []
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