骨涎蛋白(BSP)活性蛋白

Active Bone Sialoprotein (BSP)

IBSP; Bsp; BspII; SpII; Integrin-Binding Bone Sialoprotein; Cell-binding sialoprotein; Bone sialoprotein 2

  • 骨涎蛋白(BSP)活性蛋白 产品包装(模拟)
  • 骨涎蛋白(BSP)活性蛋白 产品包装(模拟)
  • APB092Ra61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Bone Sialoprotein (BSP) is a highly glycosylated and phosphorylated non-collagenous protein that plays a pivotal role in mineralized tissues, particularly in bone and dentin. As a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family, it is primarily synthesized by osteoblasts, odontoblasts, and chondrocytes, and is prominently localized in the extracellular matrix of mineralizing tissues. Additionally, it also has the function of cell adhesion. Therefore, we measure the activity of recombinant rat BSP by the ability of the immobilized protein to support the adhesion of MDA-MB-231 cells. When 5 x 104 cells/well are added to different concentrations of recombinant rat BSP coated plates, cells will adhere after 2 hour incubation at 37 ℃. The adhesion of MDA-MB-231 cells after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.48 μg/ml.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
UAPB092Ra61 骨涎蛋白(BSP)活性蛋白 Cell culture; Activity Assays.

参考文献

杂志 参考文献
FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells [Pubmed:25542352]
Journal of Dental Research Adiponectin Promotes Human Jaw Bone Marrow Stem Cell Osteogenesis [Pubmed:26961489]
circulation journal Stenotic Bicuspid and Tricuspid Aortic Valves　- Micro-Computed Tomography and Biological Indices of Calcification. [pubmed:28344201]
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