Carnitine Palmitoyltransferase 2(CPT2) is an enzyme that participates in fatty acid oxidation. It plays a crucial role in the transport of long-chain fatty acids into the mitochondria for beta-oxidation, which is the process that the body uses to break down these fatty acids to produce energy.Besides,Carnitine Palmitoyltransferase 1A(CPT1A) has been identified as an interactor of CPT2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CPT2 and recombinant human CPT1A. Briefly, CPT2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CPT1A-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CPT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat CPT2 and recombinant human CPT1A was shown in Figure 1, The binding activity of 777 and 000 was shown in Figure 1, and this effect was in a dose dependent manner.