补体成分1q子成分C(C1qC)活性蛋白
Active Complement Component 1, Q Subcomponent C (C1qC)
C1QG; Complement Component 1 q subcomponent,Gamma Polypeptide
- 编号UAPE869Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点7.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 778 ¥ 1944 ¥ 3888 ¥ 11664 ¥ 29160
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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WB图
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Complement component 1Q subcomponent C (C1qC) is an important part of the C1 complex in the classical activation pathway of the complement system. C1qC contains multiple domains that recognize and bind to pathogen surfaces or antibody-antigen complexes.C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. Thus a functional ELISA assay was conducted to detect the interaction of recombinant human C1qC and recombinant mouse C1r. Briefly, C1qC was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to C1r-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C1qC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human C1qC and recombinant mouse C1r was shown in Figure 1, the EC50 for this effect is 0.049ug/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| URPE869Hu01 | 补体成分1q子成分C(C1qC)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPE869Hu01 | 补体成分1q子成分C(C1qC)活性蛋白 | Cell culture; Activity Assays. |
| UPAE869Hu01 | 补体成分1q子成分C(C1qC)多克隆抗体 | WB; IHC; ICC; IP. |
| UMAE869Hu21 | 补体成分1q子成分C(C1qC)单克隆抗体 | WB; IHC; ICC; IP. |
| USEE869Hu | 补体成分1q子成分C(C1qC)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCE869Hu | 补体成分1q子成分C(C1qC)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULME869Hu | 补体成分1q子成分C(C1qC)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| PLOS ONE | Increased complement C1q level marks active disease in human tuberculosis [Pubmed:24647646] |
