补体因子D(CFD)活性蛋白

Active Complement Factor D (CFD)

CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator

  • 补体因子D(CFD)活性蛋白 产品包装(模拟)
  • 补体因子D(CFD)活性蛋白 产品包装(模拟)
  • APB833Ra01.jpg Figure. SDS-PAGE
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活性实验

Complement Factor D (CFD) is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3. Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity. The full-length (amino acid residues 1-263) of rat CFD was expressed which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 50 mM Tris, 1 M NaCl, pH 7.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of CFD (diluted by Assay Buffer) to 50 µl substrate mixture of 0.2mM Z-Lys-SBzl•HCl and 0.2 mM DTNB. The final well serves as a negative control with no CFD, replaced with 50 μl assay buffer. Then read in kinetic mode for 5 minutes at an absorbance of 405 nm. The specific activity of recombinant rat CFD is > 10000 pmol/min/µg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APB833Ra01 补体因子D(CFD)活性蛋白 Cell culture; Activity Assays.
RPB833Ra01 补体因子D(CFD)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB833Ra01 补体因子D(CFD)多克隆抗体 WB; IHC; ICC; IP.
MAB833Ra21 补体因子D(CFD)单克隆抗体 WB; IHC; ICC; IP.
SEB833Ra 补体因子D(CFD)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB833Ra 补体因子D(CFD)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Journal of Diabetes Investigation Relationship between serum adipsin and the first phase of glucose‐stimulated insulin secretion in individuals with different glucose tolerance [Pubmed:29432659]
Endocrine Practice CORRELATION OF INCREASED SERUM ADIPSIN WITH INCREASED CARDIOVASCULAR RISKS IN ADULT PATIENTS WITH GROWTH HORMONE DEFICIENCY [Pubmed: 30657359]
BMC Endocrine Disorders Association between plasma adipsin level and mild cognitive impairment in Chinese patients with type 2 diabetes: a cross-sectional study [Pubmed: 31651303]
Scientific Reports HFD-induced hepatic lipid accumulation and inflammation are decreased in Factor D deficient mouse [Pubmed: 33067533]
Biomedicine & Pharmacotherapy Body fat distribution and circulating adipsin are related to metabolic risks in adult patients with newly diagnosed growth hormone deficiency and improve after … [Pubmed: 33254428]
Nephrol Dial Transplant Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis [33367879]
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