甘丙肽(GAL)活性蛋白

Active Galanin (GAL)

GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide

  • 甘丙肽(GAL)活性蛋白 产品包装(模拟)
  • 甘丙肽(GAL)活性蛋白 产品包装(模拟)
  • APB084Hu02.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Galanin (GAL) is a neuropeptide widely distributed in the central and peripheral nervous systems. It consists of 29 or 30 amino acids and is involved in various physiological processes like regulating appetite, pain sensation, memory, and endocrine function.Besides,Galanin Like Peptide (GALP) has been identified as an interactor of GAL, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GAL and recombinant rat GALP. Briefly, biotin-linked GAL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to GALP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of GAL and GALP was shown in Figure 1, the EC50 for this effect is 0.282 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
UAPB084Hu02 甘丙肽(GAL)活性蛋白 Cell culture; Activity Assays.
URPB084Hu02 甘丙肽(GAL)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPB084Hu01 甘丙肽(GAL)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UCPB084Hu21 甘丙肽(GAL)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
UPAB084Hu01 甘丙肽(GAL)多克隆抗体 WB; IHC; ICC; IP.
ULAB084Hu81 甘丙肽(GAL)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
ULAB084Hu71 甘丙肽(GAL)多克隆抗体(生物素标记) WB; IHC; ICC.
UMAB084Hu22 甘丙肽(GAL)单克隆抗体 WB; IHC; ICC; IP.
UCEB084Hu 甘丙肽(GAL)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
UCCB084Hu 甘丙肽(GAL)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
ULMB084Hu 甘丙肽(GAL)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
UKSB084Hu11 甘丙肽(GAL)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Progress in Neuro-Psychopharmacology and Biological Psychiatry Decreased galanin serum levels are associated with alcohol-craving during withdrawal [ScienceDirect: S0278584610005191]
Clinical Biochemistry Circulating galanin levels are increased in patients with gestational diabetes mellitus [PubMed: 23266294]
Peptides Endogenous galanin as a novel biomarker to predict gestational diabetes mellitus. [Pubmed: 24503374]
Gynecological Endocrinology Circulating galanin and IL-6 concentrations in gestational diabetes mellitus [Pubmed:24397394]
Eat Behav Association between circulating levels of galanin and pre-pregnancy body mass index in patients with gestational diabetes mellitus [PubMed: 26172564]
Cell Biochemistry and Biophysics The Effects of Levetiracetam on Cerebrospinal Fluid and Plasma NPY and GAL, and on the Components of Stress Response System, hs-CRP, and S100B Protein in Serum of Patients with Refractory Epilepsy [Article: 10.1007]
Journal of Addiction & Prevention Low Galanin Serum Levels are Associated with Anxiety in Opioid-Maintained Patients [688687ca8d5a1d03ed060f761328001ca673.pdf]
Oncology Letter Colorectal cancer patients exhibit increased levels of galanin in serum and colon tissues [pubmed:27899999]
Scientific Reports Serum Galanin Levels in Young Healthy Lean and Obese Non-Diabetic Men during an OralGlucose Tolerance Test. [pubmed:27550417]
Journal of Clinical Investigation  Adrenomedullin and Galanin Responses to Orthostasis in Older Persons [pubmed:28796366]
Child Psychiatry Hum Dev Serum Galanin in Children with Autism Spectrum Disorder [33534034]
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