中性葡糖苷酶αAB(GaNAB)活性蛋白

Active Glucosidase Alpha, Neutral AB (GaNAB)

GluII; G2AN; Alpha-glucosidase 2; Glucosidase II subunit alpha

  • 中性葡糖苷酶αAB(GaNAB)活性蛋白 产品包装(模拟)
  • 中性葡糖苷酶αAB(GaNAB)活性蛋白 产品包装(模拟)
  • APD188Hu01.jpg Figure. SDS-PAGE
  • 中性葡糖苷酶αAB(GaNAB)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Glucosidase Alpha, Neutral AB (GaNAB) is an enzyme in the glycoside hydrolase family. It hydrolyzes alpha - glucosidic bonds, participates in carbohydrate metabolism, and is involved in endoplasmic reticulum quality control. GaNAB binds to Heat Shock 70kDa Protein 5 (HSPA5), collaborating to ensure proper glycoprotein folding and maintain cellular protein homeostasis.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GaNAB and recombinant mouse HSPA5 .Briefly, biotin-linked GaNAB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to HSPA5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of GaNAB and HSPA5 was shown in Figure 1, the EC50 for this effect is 3.21ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
UAPD188Hu01 中性葡糖苷酶αAB(GaNAB)活性蛋白 Cell culture; Activity Assays.
URPD188Hu01 中性葡糖苷酶αAB(GaNAB)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAD188Hu01 中性葡糖苷酶αAB(GaNAB)多克隆抗体 WB; IHC; ICC; IP.
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