谷胱甘肽S转移酶κ1(GSTk1)活性蛋白
Active Glutathione S Transferase Kappa 1 (GSTk1)
GST-K1; GST13; HDCMD47P; Glutathione S-transferase subunit 13
- 编号UAPB089Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点7.7
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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活性实验

Glutathione S Transferase Kappa 1 (GSTk1) is a member of the GST superfamily. It has unique enzymatic activities and plays important roles in cellular detoxification and protection against oxidative stress. GSTk1 is involved in conjugating glutathione to a variety of substrates, facilitating the removal of harmful substances from cells. It is expressed in different tissues and has been implicated in various physiological and pathological processes. Its functions are crucial for maintaining cellular homeostasis and overall organismal health.Besides,the binding of GSTk1 and CDK2 may regulate cell cycle progression and affect cell proliferation, potentially influencing tumor development and other cellular events.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human GSTk1 and recombinant mouse CDK2. Briefly, GSTk1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CDK2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSTk1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human GSTk1 and recombinant mouse CDK2 was shown in Figure 1, the EC50 for this effect is 0.046ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
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相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
UAPB089Hu01 | 谷胱甘肽S转移酶κ1(GSTk1)活性蛋白 | Cell culture; Activity Assays. |
URPB089Hu01 | 谷胱甘肽S转移酶κ1(GSTk1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
UPAB089Hu01 | 谷胱甘肽S转移酶κ1(GSTk1)多克隆抗体 | WB; IHC |
UMAB089Hu22 | 谷胱甘肽S转移酶κ1(GSTk1)单克隆抗体 | WB; IHC; ICC; IP. |
USEB089Hu | 谷胱甘肽S转移酶κ1(GSTk1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |