颗粒酶A(GZMA)活性蛋白
Active Granzyme A (GZMA)
CTLA3; HFSP; HF; H factor; Granzyme 1; Fragmentin-1; Hanukkah factor; Cytotoxic T-Lymphocyte-Associated Serine Esterase 3; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase independent cell death when introduced into target cells by perforin (1). Mouse granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1 26), a propeptide (residues 27-28) and a mature chain (residues 29-262). The purified recombinant mouse Granzyme A consists of residues 29 to 262 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (Assay Buffer), initiated by addition 50 μL of various concentrations of GZMA (dilute by Assay Buffer) to 50 µL of 1.2 mM Substrate and DTNB mixture. The final well serves as a negative control with no GZMA, replace with 50μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant mouse Granzyme A is 6214 pmol/min/µg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| URPA599Mu01 | 颗粒酶A(GZMA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA599Mu02 | 颗粒酶A(GZMA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA599Mu61 | 颗粒酶A(GZMA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA599Mu61 | 颗粒酶A(GZMA)活性蛋白 | Cell culture; Activity Assays. |
| UPAA599Mu02 | 颗粒酶A(GZMA)多克隆抗体 | WB; IHC; ICC; IP. |
| UPAA599Mu01 | 颗粒酶A(GZMA)多克隆抗体 | WB |
| ULAA599Mu71 | 颗粒酶A(GZMA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| USEA599Mu | 颗粒酶A(GZMA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA599Mu | 颗粒酶A(GZMA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| UKSA599Mu01 | 颗粒酶A(GZMA)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Cell Reports | Granzyme A Produces Bioactive IL-1β through a Nonapoptotic Inflammasome-Independent Pathway [Pubmed:25437548] |
| Journal of Dermatological Science | Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopicdermatitis. [pubmed:27686401] |
| Journal of Maternal-Fetal & Neonatal Medicine | Extracellular granzyme A in amniotic fluid is elevated in the presence of sterile intra-amniotic inflammation in preterm prelabor rupture of membranes [Pubmed: 32912008] |
