次黄嘌呤磷酸核糖基转移酶1(HPRT1)活性蛋白

Active Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)

HGPRT; HGPRTase; HPRT; Lesch-Nyhan Syndrome; Hypoxanthine-guanine phosphoribosyltransferase

  • 次黄嘌呤磷酸核糖基转移酶1(HPRT1)活性蛋白 产品包装(模拟)
  • 次黄嘌呤磷酸核糖基转移酶1(HPRT1)活性蛋白 产品包装(模拟)
  • APA717Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) is an enzyme central to the purine salvage pathway. It utilizes phosphoribosylpyrophosphate (PRPP) to transform hypoxanthine into inosine monophosphate (IMP) and guanine into guanosine monophosphate (GMP). APRT, on the other hand, catalyzes the conversion of adenine to adenosine monophosphate (AMP) also with PRPP. When HPRT1 and APRT interact, they can potentially co - ordinate the utilization of PRPP.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human HPRT1 and recombinant human APRT. Briefly, HPRT1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APRT-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HPRT1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human HPRT1 and recombinant human APRT was shown in Figure 1, the EC50 for this effect is 0.092ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
UAPA717Hu01 次黄嘌呤磷酸核糖基转移酶1(HPRT1)活性蛋白 Cell culture; Activity Assays.
URPA717Hu01 次黄嘌呤磷酸核糖基转移酶1(HPRT1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAA717Hu01 次黄嘌呤磷酸核糖基转移酶1(HPRT1)多克隆抗体 WB,IHC,FCM
UFAA717Hu01 抗次黄嘌呤磷酸核糖基转移酶1(HPRT1)多克隆抗体 FCM
UMAA717Hu22 次黄嘌呤磷酸核糖基转移酶1(HPRT1)单克隆抗体 WB; IHC; ICC; IP.
USEA717Hu 次黄嘌呤磷酸核糖基转移酶1(HPRT1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA717Hu 次黄嘌呤磷酸核糖基转移酶1(HPRT1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
J Crohns Colitis. Response to infliximab therapy in ulcerative colitis is associated with decreased monocyte activation, reduced CCL2 expression and downregulation of Tenascin C. [Pubmed:25518051]
J Crohns Response to infliximab therapy in ulcerative colitis is associated with decreased monocyte activation, reduced CCL2 expression and downregulation of tenascin C [PubMed: 25518051]
Molecular Biology Reports Increased ENT2 Expression and Its Association With Altered Purine Metabolism in Different Stages of Colorectal Cancer Cell Lines []
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