白介素34(IL34)活性蛋白

Active Interleukin 34 (IL34)

C16orf77

  • 白介素34(IL34)活性蛋白 产品包装(模拟)
  • 白介素34(IL34)活性蛋白 产品包装(模拟)
  • APC007Hu63.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Interleukin 34 (IL-34) is a protein belonging to a group of cytokines called interleukins. IL-34 increases growth or survival of immune cells known as monocytes; it elicits its activity by binding the Colony stimulating factor 1 receptor. To test the effect of IL-34 on cell proliferation, TE-1 cells were seeded into triplicate wells of 96-well plates which contains various concentrations of recombinant human IL-34. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 ℃. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay. The result was shown in Figure 1. It was obvious that IL-34 significantly increased cell viability of TE-1 cells. The ED50 is 2.252 µg/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
UAPC007Hu61 白介素34(IL34)活性蛋白 Cell culture; Activity Assays.
UAPC007Hu01 白介素34(IL34)活性蛋白 Cell culture; Activity Assays.
URPC007Hu02 白介素34(IL34)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPC007Hu63 白介素34(IL34)活性蛋白 Cell culture; Activity Assays.
UEPC007Hu62 白介素34(IL34)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPC007Hu01 白介素34(IL34)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAC007Hu01 白介素34(IL34)多克隆抗体 WB; IHC; ICC; IP.
UMAC007Hu21 白介素34(IL34)单克隆抗体 WB
UMAC007Hu23 白介素34(IL34)单克隆抗体 WB
UMAC007Hu22 白介素34(IL34)单克隆抗体 WB
USEC007Hu 白介素34(IL34)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
USCC007Hu 白介素34(IL34)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
ULMC007Hu 白介素34(IL34)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
J Int Med Res. Increased Serum Interleukin-34 in Patients with Coronary Artery Disease [PubMed: 23206468]
Transplantation IL-34 inhibits acute rejection of rat liver transplantation by inducing Kupffer cell M2 polarization [Pubmed:29570162]
cyberleninka КЛИНИКО-ЭПИДЕМИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА И ОСОБЕННОСТИ БАЛЛАНСА ЦИТОКИНОВ ПРИ КЛЕЩЕВОМ ЭНЦЕФАЛИТЕ В … []
Revista da Associacao Medica Brasileira A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic … []
Rev Assoc Med Bras A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡­ [33295414]
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