脂蛋白关联磷脂酶A2(LpPLA2)活性蛋白

Active Phospholipase A2 Group VII (LpPLA2)

PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2; Phospholipase A2, Lipoprotein Associated

  • 脂蛋白关联磷脂酶A2(LpPLA2)活性蛋白 产品包装(模拟)
  • 脂蛋白关联磷脂酶A2(LpPLA2)活性蛋白 产品包装(模拟)
  • 脂蛋白关联磷脂酶A2(LpPLA2)活性蛋白 Figure. Gene Sequencing (Extract)
  • APA867Hu01.png Figure. SDS-PAGE
  • 脂蛋白关联磷脂酶A2(LpPLA2)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Phospholipase A2 Group VII (PLA2G7) which is also known as Lp-PLA2, is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%-20% to HDL, and the remainder to VLDL. It is produced in major by mature macrophages and activated platelets and catalyzes the degradation of platelet-activating factor to biologically inactive products. Besides, Paraoxonase 1 (PON1) has been identified as an interactor of PLA2G7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human PLA2G7 and recombinant rat PON1. Briefly, PLA2G7 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PON1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PLA2G7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PLA2G7 and recombinant rat PON1 was shown in Figure 1, the EC50 for this effect is 0.7 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
URPA867Hu02 脂蛋白关联磷脂酶A2(LpPLA2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPA867Hu03 脂蛋白关联磷脂酶A2(LpPLA2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPA867Hu04 脂蛋白关联磷脂酶A2(LpPLA2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPA867Hu01 脂蛋白关联磷脂酶A2(LpPLA2)活性蛋白 Cell culture; Activity Assays.
URPA867Hu01 脂蛋白关联磷脂酶A2(LpPLA2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAA867Hu01 脂蛋白关联磷脂酶A2(LpPLA2)多克隆抗体 WB
ULAA867Hu81 脂蛋白关联磷脂酶A2(LpPLA2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
ULAA867Hu71 脂蛋白关联磷脂酶A2(LpPLA2)多克隆抗体(生物素标记) WB; IHC; ICC.
UMAA867Hu21 脂蛋白关联磷脂酶A2(LpPLA2)单克隆抗体 WB; IHC; ICC; IP.
UMAA867Hu22 脂蛋白关联磷脂酶A2(LpPLA2)单克隆抗体 WB; IHC; ICC; IP.
USEA867Hu 脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
USCA867Hu 脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
ULMA867Hu 脂蛋白关联磷脂酶A2(LpPLA2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Science & Sports The effects of different intensity walking programs on serum blood lipids, high-sensitive C-reactive protein, and lipoprotein-associated phospholipase A2 in premenopausal women [ScienceDirect: S0765159710000262]
Metabolic Syndrome and Related Disorders Association of Serum Lipoprotein-Associated Phospholipase A2 Level with Nonalcoholic Fatty Liver Disease [Wiley: source]
34 Effects of Pycnogenol on endothelial function in patients with stable coronary artery disease: a double-blind, randomized, placebo-controlled, cross-over study [PubMed: 22240497]
PLoS ONE Effects of Lipid-Lowering Drugs on Irisin in Human Subjects In Vivo and in Human Skeletal Muscle Cells Ex Vivo [PubMed: PMC3759413]
China Pharmacy Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats [Source]
Atherosclerosis Everolimus therapy is associated with reduced lipoprotein-associated phospholipase A2 (Lp-Pla2) activity and oxidative stress in heart transplant recipients. [Pubmed: 23958269]
Journal of Diabetes Research Lipoprotein-Associated Phospholipase A2 Mass Level Is Increased in Elderly Subjects with Type 2 Diabetes Mellitus [Hindawi: 278063]
Health The Role of Asymmetric Dimethylarginine and Lipoprotein Associated Phospholipase A2 in Children and Adolescents with Dyslipidemia [Scirp:Source]
Biology Bulletin In vitro lipid-lowering and fibrinolytic effects of regulatory leucine-containing glyprolines in human blood [Springer:Source]
Gynecol Endocrinol. Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. [Pubmed:24397392]
Journal of Diabetes Research Lipoprotein-Associated Phospholipase A 2 Mass Level Is Increased in Elderly Subjects with Type 2 Diabetes Mellitus [Pubmed:24818163]
Metabolism. Effects of sitagliptin therapy on markers of low-grade inflammation and cell adhesion molecules in patients with type 2 diabetes [Pubmed:25034387]
J Am Heart Assoc Effect of Extended‐Release Niacin on High‐Density Lipoprotein (HDL) Functionality, Lipoprotein Metabolism, and Mediators of Vascular Inflammation in Statin‐Treated Patients [PubMed: 26374297]
Czecho-Slovak Dermatology / Cesko-Slovenska Dermatologie V [Ebscohost]
Endocrinol Metab (Seoul). Effect of Pitavastatin Treatment on ApoB-48 and Lp-PLA2 in Patients with Metabolic Syndrome: Substudy of PROspective Comparative Clinical Study Evaluating the Efficacy and Safety of PITavastatin in Patients with Metabolic Syndrome [pmc:PMC4803547]
Clin Pharmacol Ther.  Effect of dipyridamole on myocardial reperfusion injury: A double‐blind randomized controlled trial in patients undergoing elective coronary artery bypass surgery [Pubmed:25773594]
Lipids in Health and Disease Effect of intensive insulin treatment on plasma levels of lipoprotein-associated phospholipase A 2 and secretory phospholipase A 2 in patients with newly … [articles:10.1186]
Acta Endocrinologica SERUM LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 IN MALES WITH METABOLIC SYNDROME AND OBSTRUCTIVE SLEEP APNEA. [jrnl:1841098]
Biomarker for diagnosis of moyamoya disease [:]
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION Circulating fatty-acid binding-protein 4 levels predict CV events in patients after coronary interventions [Pubmed: 32859455]
chinese neurosurgical journal Can admission lipoprotein-associated phospholipase A2 predict the symptomatic cerebral vasospasm following aneurysmal subarachnoid hemorrhage? []
留言咨询