纤溶酶原激活物抑制因子2(PAI2)活性蛋白
Active Plasminogen Activator Inhibitor 2 (PAI2)
SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor
- 编号APA531Mu61
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点4.8
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
Serpin B2, also known as PAI-2, is an approximately 60 kDa serine protease inhibitor. It is primarily secreted by macrophages and monocytes and can form disulfide-linked multimers. Serpin B2 inhibits both the urokinase-type and tissue-type plasminogen activators (uPA and tPA). Serpin B2 also promotes the clearance of uPA by enhancing its binding and uptake by LRP. It limits fibril formation by Huntington protein (HTT) and beta-Amyloid peptides. It promotes Th2 biased immune responses and is important for intestinal CCL2 production, monocyte recruitment, and nematode clearance. A non-glycosylated form of Serpin B2 is retained intracellularly where it interferes with TNF-a induced apoptosis by protecting the Retinoblastoma protein (RB1) from calpain digestion. It also inhibits proteasome activity in activated endothelial cells. The activity of recombinant mouse PAI-2 was measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) in the assay buffer 50 mM Tris, 0.01% Tween 20, pH 8.5. The 50 ul different concentrations of rmPAI-2 (MW: 47.9 KD) was incubated with 50ul 2ug/ml rhuPA (EPA140Mu61) at room temperature for 15 minutes. Loading 50 µL of the incubated mixtures into empty wells of a plate, and start the reaction by adding 50 µL of 200 µM substrate (Z-GGR-AMC). Include a substrate blank containing 50 µL of assay buffer and 50 µL of 200 µM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that PAI2 significantly decreased uPA activity. The inhibition IC50 was <300 nM.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
APA531Mu61 | 纤溶酶原激活物抑制因子2(PAI2)活性蛋白 | Cell culture; Activity Assays. |
RPA531Mu02 | 纤溶酶原激活物抑制因子2(PAI2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA531Mu61 | 纤溶酶原激活物抑制因子2(PAI2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA531Mu01 | 纤溶酶原激活物抑制因子2(PAI2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA531Mu01 | 纤溶酶原激活物抑制因子2(PAI2)多克隆抗体 | WB; IHC; ICC; IP. |
PAA531Mu02 | 纤溶酶原激活物抑制因子2(PAI2)多克隆抗体 | WB; IHC; ICC; IP. |
SEA531Mu | 纤溶酶原激活物抑制因子2(PAI2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA531Mu | 纤溶酶原激活物抑制因子2(PAI2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
BBA Clinical | Cerebrospinal fluid inflammatory markers in patients with?Listeria monocytogenes?meningitis [Sciencedirect:S2214647414000087] |
JCI Insight | The senescence-associated secretome as an indicator of age and medical risk [Pubmed: 32554926] |