前脑啡肽(PENK)活性蛋白
Active Proenkephalin (PENK)
Preproenkephalin; Proenkephalin-A; Synenkephalin; Met-enkephalin; Met-enkephalin-Arg-Gly-Leu; Leu-enkephalin; Met-enkephalin-Arg-Phe
- 编号UAPD396Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点5.3
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
Figure. SDS-PAGE
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
Proenkephalin (PENK) is a protein encoded by the PENK gene. It is initially synthesized as a preproprotein and undergoes proteolytic processing to generate multiple products. As PENK has the function of inhibit the migration cells，we measure the activity of recombinant mouse PENK by the ability of the protein to inhibit the migration of U2OS cells. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the inhibition of chemotactic effect of recombinant mouse PENK on U2OS cell line. Briefly, U2OS cells were seeded into the upper chambers and PENK was added in lower chamber with a polycarbonate filter (8 μm pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×10) and the number of migrated cells were counted randomly (five fields for each filter). Result shows MIP-3α is able to induce migration of U2OS cells. The migrated U2OS cells in low chamber at low magnification (×10) were shown in Figure 1.
用法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| UAPD396Mu01 | 前脑啡肽(PENK)活性蛋白 | Cell culture; Activity Assays. |
| URPD396Mu02 | 前脑啡肽(PENK)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPD396Mu01 | 前脑啡肽(PENK)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAD396Mu01 | 前脑啡肽(PENK)多克隆抗体 | WB; IHC; ICC; IP. |
| UPAD396Mu02 | 前脑啡肽(PENK)多克隆抗体 | WB; IHC; ICC; IP. |
| USED396Mu | 前脑啡肽(PENK)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMD396Mu | 前脑啡肽(PENK)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
