S-期激酶关联蛋白1(SKP1)活性蛋白

Active S-Phase Kinase Associated Protein 1 (SKP1)

SKP1A; EMC19; OCP-II; OCP2; TCEB1L; p19A; Cyclin-A/CDK2-associated p19; RNA polymerase II elongation factor-like protein; Transcription elongation factor B polypeptide 1-like

  • S-期激酶关联蛋白1(SKP1)活性蛋白 产品包装(模拟)
  • S-期激酶关联蛋白1(SKP1)活性蛋白 产品包装(模拟)
  • APF883Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

S-Phase Kinase Associated Protein 1 (SKP1) is a core component of the SKP1-Cullin-F-box (SCF) E3 ubiquitin ligase complex. It is an adaptor protein, connecting cullin and F-box proteins in the complex. SKP1 mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Previous studies have shown that SKP1 protein is associated with Mitochondrial Dna Depletion Syndrome 13 and Parkinson. To test the effect of SKP1 on cell proliferation, A549 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human SKP1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant human SKP1 for 72h. The result was shown in Figure 1. It was obvious that SKP1 significantly decreased cell viability of A549 cells. The ED50 of recombinant human SKP1 is 0.375 μg/ml.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
URPF883Hu01 S-期激酶关联蛋白1(SKP1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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ULMF883Hu S-期激酶关联蛋白1(SKP1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
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