转化生长因子β2(TGFb2)活性蛋白

Active Transforming Growth Factor Beta 2 (TGFb2)

TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor

  • 转化生长因子β2(TGFb2)活性蛋白 产品包装(模拟)
  • 转化生长因子β2(TGFb2)活性蛋白 产品包装(模拟)
  • APA218Hu01.png Figure. SDS-PAGE
  • 转化生长因子β2(TGFb2)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Transforming Growth Factor Beta 2 (TGFB2) belongs to TGF-beta family. As a secreted protein and a cytokine, TGFB2 plays a role in the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. Besides, TGFB2 regulates cell proliferation, differentiation, adhesion, migration by transducing the signal through transmembrane receptors type I and type II (TGFBR1 and TGFBR2). To test the effect of TGFb2 on inhibit IL4-dependent proliferation, CTLL-2 cells were seeded into triplicate wells of 96-well plates, the medium was 2% serum RPMI-1640 including various concentrations of recombinant human TGFb2. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured. Cell viability was assessed by CCK-8 assay. The result was shown in Figure1. It was obvious that TGFB2 significantly decreased cell viability of CTLL-2 cells. The ED50 of recombinant human TGFB2 is 1.95μg/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
URPA218Hu01 转化生长因子β2(TGFb2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPA218Hu01 转化生长因子β2(TGFb2)活性蛋白 Cell culture; Activity Assays.
UPAA218Hu01 转化生长因子β2(TGFb2)多克隆抗体 IHC; FCM
URAA218Hu21 转化生长因子β2(TGFb2)重组抗体 WB; IF; ICC; IHC; IP; FCM.
UMAA218Hu21 转化生长因子β2(TGFb2)单克隆抗体 WB
UMAA218Hu22 转化生长因子β2(TGFb2)单克隆抗体 WB; IHC; ICC; IP.
UFAA218Hu08 抗转化生长因子β2(TGFb2)单克隆抗体 FCM
UFAA218Hu06 抗转化生长因子β2(TGFb2)单克隆抗体 FCM
UFAA218Hu04 抗转化生长因子β2(TGFb2)单克隆抗体 FCM
UFAA218Hu02 抗转化生长因子β2(TGFb2)单克隆抗体 FCM
USEA218Hu 转化生长因子β2(TGFb2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
UCEA218Hu 转化生长因子β2(TGFb2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA218Hu 转化生长因子β2(TGFb2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source]
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173]
International Journal of Chemical Studies Studies on quality attributes of skimmed colostrum powder [P-ISSN: 2349–8528]
JCI Insight Angiocrine signals regulate quiescence and therapy resistance in bone metastasis [Pubmed: 31292293]
Mol Med Association of CASC18/miR-20a-3p/TGFB2 ceRNA axis with occult lymph node metastasis in tongue squamous cell carcinoma [34362313]
Food Chem Effect of convection and microwave heating on the retention of bioactive components in human milk [34896952]
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