周期素依赖性激酶抑制因子2A(CDKN2A)多克隆抗体
Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)
P16; ARF; CDK4I; CMM2; INK4; INK4a; MLM; MTS1; TP16; P14; P14ARF; P16INK4a; P19; Melanoma P16; Cyclin-dependent kinase 4 inhibitor A; Multiple tumor suppressor 1
- 编号UPAA794Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPA794Hu01-周期素依赖性激酶抑制因子2A(CDKN2A)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.5mg/mL
- 且适物种Rattus norvegicus (Rat，大鼠)
- 应用WB; IHC; ICC/IF; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 284 ¥ 664 ¥ 948 ¥ 2370 ¥ 9480
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CDKN2A Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CDKN2A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20µg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 0.75µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant CDKN2A, Human.
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate; Lane3: HepG2 cell lysate;
Primary Ab: 0.05µg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human CDKN2A Polyclonal Antibody (Catalog # PAA794Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CDKN2A Polyclonal Antibody (Catalog PAA794Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对CDKN2A的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CDKN2A。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
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相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| URPA794Hu01 | 周期素依赖性激酶抑制因子2A(CDKN2A)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA794Hu01 | 周期素依赖性激酶抑制因子2A(CDKN2A)多克隆抗体 | WB; IHC; ICC/IF; FCM |
| UFAA794Hu01 | 抗周期素依赖性激酶抑制因子2A(CDKN2A)多克隆抗体 | FCM |
| UMAA794Hu22 | 周期素依赖性激酶抑制因子2A(CDKN2A)单克隆抗体 | WB |
| UMAA794Hu21 | 周期素依赖性激酶抑制因子2A(CDKN2A)单克隆抗体 | WB |
| UMAA794Hu23 | 周期素依赖性激酶抑制因子2A(CDKN2A)单克隆抗体 | WB |
| UMAA794Hu24 | 周期素依赖性激酶抑制因子2A(CDKN2A)单克隆抗体 | WB |
| UMAA794Hu25 | 周期素依赖性激酶抑制因子2A(CDKN2A)单克隆抗体 | WB |
| UMAA794Hu26 | 周期素依赖性激酶抑制因子2A(CDKN2A)单克隆抗体 | WB |
| UMAA794Hu27 | 周期素依赖性激酶抑制因子2A(CDKN2A)单克隆抗体 | WB |
| UMAA794Hu28 | 周期素依赖性激酶抑制因子2A(CDKN2A)单克隆抗体 | WB |
| UMAA794Hu29 | 周期素依赖性激酶抑制因子2A(CDKN2A)单克隆抗体 | WB |
| UMEA794Hu | 周期素依赖性激酶抑制因子2A(CDKN2A)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA794Hu | 周期素依赖性激酶抑制因子2A(CDKN2A)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA794Hu | 周期素依赖性激酶抑制因子2A(CDKN2A)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Biochemical genetics | Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer [pubmed:28421310] |
| European Journal of Pharmaceutical Sciences | Development of core-shell nanocarrier system for augmenting piperine cytotoxic activity against human brain cancer cell line [Pubmed:29597041] |
| Clinical and Experimental Medicine | Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetes [Pubmed: 31732824] |
| MOLECULAR AND CELLULAR BIOCHEMISTRY | Tumor suppressor genes are differentially regulated with dietary folate modulations in a rat model of hepatocellular carcinoma [Pubmed: 32974833] |
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