半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体

Polyclonal Antibody to Cystatin B (CSTB)

Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor

  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 产品包装(模拟)
  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 产品包装(模拟)
  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 DAB staining on IHC-P; Samples: Human Thyroid Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CSTB Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 DAB staining on IHC-P;
    Sample: Human Esophagus cancer Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human CSTB Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 FITC staining on IF;
    Sample: Human MCF7 cell;
    Primary Ab: 10µg/ml Rabbit Anti-Human CSTB Antibody
    Second Ab: 1.3µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAJ322Hu01.jpg Figure. Western Blot; Sample: Recombinant CSTB, Human.
  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 Knockout Varification: <br/>Lane 1: Wild-type HepG2 cell lysate; <br/>Lane 2: CSTB knockout HepG2 cell lysate; <br/>Predicted MW: 11kDa <br/>Observed MW: 14kDa<br/>Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 Western Blot; Sample: Human Liver lysate; <br/>Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: MCF7 cell lysate; Lane3: A549 cell lysate;
    Primary Ab: 0.05µg/ml Rabbit Anti-Human CSTB Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 Human A431 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CSTB Polyclonal Antibody (Catalog PAJ322Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对CSTB的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CSTB。

用法

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
For flow cytometry: 0.5-2 µg per 10^6 cells in 100 µl volume.
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPJ322Hu01 半胱氨酸蛋白酶抑制剂B(CSTB)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAJ322Hu01 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 WB;IHC;ICC/IF;FCM
MAJ322Hu21 半胱氨酸蛋白酶抑制剂B(CSTB)单克隆抗体 WB; IHC; ICC; IP.
SEJ322Hu 半胱氨酸蛋白酶抑制剂B(CSTB)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ322Hu 半胱氨酸蛋白酶抑制剂B(CSTB)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
AIDS. Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders [PubMed: 23291538]
International Journal of Circumpolar Health Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer [PubMed: PMC3754495]
PLoS One. Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis [PubMed: PMC3365072]
Journal of Neuroimmune Pharmacology Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages [Pubmed:25209871]
European Journal of Cancer Supplements A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system [Science: Article]
Annals of Clinical Biochemistry Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis. [pubmed:28766361]
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