谷氨酰胺酶(GLS)多克隆抗体
Polyclonal Antibody to Glutaminase (GLS)
GLS1; Glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase
- 编号UPAJ026Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPJ026Mu01-谷氨酰胺酶(GLS)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.5mg/mL
- 且适物种Homo sapiens (Human，人)
- 应用WB; IHC
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 324 ¥ 756 ¥ 1080 ¥ 2700 ¥ 10800
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse GLS Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: 293T cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse GLS Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Skeletal muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse GLS Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant GLS, Mouse.
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Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: GLS knockout 293T cell lysate;
Predicted MW: 73,65kd
Observed MW: 60kd
Primary Ab: 2µg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对GLS的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GLS。
用法
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| URPJ026Mu01 | 谷氨酰胺酶(GLS)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPJ026Mu02 | 谷氨酰胺酶(GLS)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAJ026Mu02 | 谷氨酰胺酶(GLS)多克隆抗体 | WB; IHC; ICC; IP. |
| UPAJ026Mu01 | 谷氨酰胺酶(GLS)多克隆抗体 | WB; IHC |
| ULAJ026Mu41 | 谷氨酰胺酶(GLS)多克隆抗体(藻红素标记) | WB |
| USEJ026Mu | 谷氨酰胺酶(GLS)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMJ026Mu | 谷氨酰胺酶(GLS)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| UCMJ026Mu | 谷氨酰胺酶(GLS)等多因子检测试剂盒(流式荧光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Neuroscience | Sex differences in the glutamate signaling pathway in juvenile rats [Pubmed:28861894] |
| Journal of Affective Disorders | Glutamatergic hypofunction in medication-free major depression: Secondary effects of affective diagnosis and relationship to peripheral glutaminase [Pubmed:29544167] |
| 校刊 | King's Research Portal [Doi: 10.1016/j.jad.2018.02.059] |
| bmc research notes | The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers [Pubmed: 32698888] |
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