血管内皮生长因子A(VEGFA)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Vascular Endothelial Growth Factor A (VEGFA)
VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
- 编号USCA143Ra
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长2h, 40min
- 检测范围2.74-2,000pg/mL
- 灵敏度最小可检测剂量小于等于0.99pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 1915 ¥ 2736 ¥ 12312 ¥ 23256 ¥ 191520
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血管内皮生长因子A(VEGFA)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血管内皮生长因子A(VEGFA)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 97 |
| EDTA plasma(n=5) | 97-105 | 101 |
| heparin plasma(n=5) | 95-102 | 99 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血管内皮生长因子A(VEGFA)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管内皮生长因子A(VEGFA)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-105% | 79-88% | 85-101% | 92-102% |
| EDTA plasma(n=5) | 86-101% | 88-101% | 78-92% | 95-102% |
| heparin plasma(n=5) | 95-102% | 85-92% | 88-96% | 89-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分钟；
8. 读数。
实验原理
将血管内皮生长因子A(VEGFA)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管内皮生长因子A(VEGFA)与连接于固相载体上的抗体结合，然后加入生物素化的血管内皮生长因子A(VEGFA)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的血管内皮生长因子A(VEGFA)呈正相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| URPA143Ra01 | 血管内皮生长因子A(VEGFA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA143Ra61 | 血管内皮生长因子A(VEGFA)活性蛋白 | Cell culture; Activity Assays. |
| UEPA143Ra61 | 血管内皮生长因子A(VEGFA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA143Ra01 | 血管内皮生长因子A(VEGFA)活性蛋白 | Cell culture; Activity Assays. |
| URPA143Ra02 | 血管内皮生长因子A(VEGFA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA143Ra02 | 血管内皮生长因子A(VEGFA)多克隆抗体 | IHC |
| UPAA143Ra01 | 血管内皮生长因子A(VEGFA)多克隆抗体 | WB; IHC; ICC/IF |
| UFAA143Ra01 | 抗血管内皮生长因子A(VEGFA)多克隆抗体 | FCM |
| ULAA143Ra81 | 血管内皮生长因子A(VEGFA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| ULAA143Ra71 | 血管内皮生长因子A(VEGFA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| UMAA143Ra21 | 血管内皮生长因子A(VEGFA)单克隆抗体 | WB; ICC/IF |
| UMAA143Ra23 | 血管内皮生长因子A(VEGFA)单克隆抗体 | WB |
| UMAA143Ra22 | 血管内皮生长因子A(VEGFA)单克隆抗体 | WB; IHC |
| UMEA143Ra | 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA143Ra | 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA143Ra | 血管内皮生长因子A(VEGFA)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA143Ra | 血管内皮生长因子A(VEGFA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| UKSA143Ra01 | 血管内皮生长因子A(VEGFA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care [33469783] |
| Advanced Healthcare Materials | Accelerated Bone Regeneration by MOF Modified Multifunctional Membranes through Enhancement of Osteogenic and Angiogenic Performance [33448103] |
| Aesthet Surg J | Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water [33428732] |
| INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | In-vivo evaluation of alginate-pectin hydrogel film loaded with simvastatin for diabetic wound healing in Streptozotocin-induced diabetic rats [33421467] |
| Eur J Pharmacol | Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ¡ [33705802] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473] |
| Journal of Tissue Engineering | Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders [33868627] |
| EXPERIMENTAL EYE RESEARCH | Astaxanthin mediated regulation of VEGF through HIF1¦Á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model [33789142] |
| Randomized Controlled Trial | Effect of Freeze-Dried Amnion Graft on Cytokines in Uterine Exudates Following Hysteroscopic Adhesiolysis of Severe Intrauterine Adhesions [28170094] |
| cancer communications | Programmed death ligand©\1 regulates angiogenesis and metastasis by participating in the c©\JUN/VEGFR2 signaling axis in ovarian cancer [33939321] |
| Öйú¼ÄÉú³æѧÓë¼ÄÉú³æ²¡ÔÓÖ¾ | VEGFA/VEGFR2 ÔÚСÊó¸Î¶à·¿¼¬ÇòòÊ×é֯Ѫ¹ÜÉú³ÉÖеıí´ï¼°×÷Óà [] |
| Chinese Journal of Parasitology and parasitic diseases | Expression and angiogenic effect of VEGFA/VEGFR2 in mice hepatic metacestode tissue of Echinococcus multilocularis [] |
| International Journal of Life science and Pharma Research | Artificial Sweeteners Alter Early Vitelline Vessel Development in Chick Embryo.(2021) [] |
| Biomed Mater | Preclinical efficacy study of a porous biopolymeric scaffold based on (gelatin-hyaluronic acid-chondroitin sulfate) in a porcine burn injury model: Role of critical … [34384056] |
| J Cell Mol Med | Mesenchymal stem cells from different sources show distinct therapeutic effects in hyperoxia‐induced bronchopulmonary dysplasia in rats [34322990] |
| J Biochem Mol Toxicol | Thymoquinone upregulates miR‐125a‐5p, attenuates STAT3 activation, and potentiates doxorubicin antitumor activity in murine solid Ehrlich carcinoma [34605108] |
| Bioengineered | MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2: Running title: miR934 … [34699325] |
| ACS Biomater Sci Eng | Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum [34472855] |
| Cells | A-Kinase Anchoring Protein 2 Promotes Protection against Myocardial Infarction [34831084] |
| J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats [Pubmed:34986543] |
| Stem Cell Res Ther | A dual role of HIF1α in regulating osteogenesis–angiogenesis coupling [Pubmed:35123567] |
| American Journal of Translational Research | Curcumin treats endometriosis in mice by the HIF signaling pathway [Pubmed:35559378] |
| Clinical and Experimental Immunology | Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients [Pubmed:35348620] |
| Medicina | Assessment of the RANTES Level Correlation and Selected Inflammatory and Pro-Angiogenic Molecules Evaluation of Their Influence on CRC Clinical Features: A … [Pubmed:35208526] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats [] |
| Research Square | DMOG attenuates steroid-associated endothelial progenitor cell impairment and osteonecrosis of the femoral head via regulating HIF-1α signaling pathway in … [] |
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