α-生育酚(TCPa)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Alpha-Tocopherol (TCPa)

VE; Vitamin E; α-TCP; α-Tocopherol

  • α-生育酚(TCPa)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • α-生育酚(TCPa)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对α-生育酚(TCPa)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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KSA922Ge11 α-生育酚(TCPa)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
International Journal of Advanced Research Evaluation of enzymatic and non- enzymatic antioxidant status in seminal plasma of Iraqi Infertile Men [Com:Source]
Journal of Physiology and Pharmacology Effect of vitamin E on cerebral cortical oxidative stress and brain-derived neurotrophic factor gene expression induced by hypoxia and exercise in rats. [PubMed: 25903950]
Mycotoxin Res  The potential effects of antioxidant feed additives in mitigating the adverse effects of cornnaturally contaminated with Fusarium mycotoxins on antioxidant systems in the intestinal mucosa, plasma, and liver in weaned pigs. [Pubmed:27021614]
The Journal of Nutritional Biochemistry Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genes [pubmed:27816814]
Plant Physiol Biochem Ultrasonication of in vitro potato single node explants: Activation and recovery of antioxidant defence system and growth responses [pubmed:29102903]
PLoS One Oxidative stress in lung cancer patients is associated with altered serum markers of lipid metabolism [Pubmed: 30973911]
Prooxidant effect of α-tocopherol in pancreatic tumors. []
Vitamin A, C, D, E and B12 Levels in Leprosy: A Case Control Study []
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