前梯度蛋白2(AGR2)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Anterior Gradient 2 (AGR2)

GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Anterior Gradient Protein 2; Protein Disulfide Isomerase Family A,Member 17; Secreted cement gland protein XAG-2 homolog

  • 前梯度蛋白2(AGR2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 前梯度蛋白2(AGR2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对前梯度蛋白2(AGR2)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Cancer Biomarkers Serum AGR2 as an early diagnostic and postoperative prognostic biomarker of human lung adenocarcinoma [MetaPress: n7137r6271u54118]
Molecular & Cellular Proteomics Integrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer Biomarkers [PubMed: PMC3205865]
Prostate. Anterior gradient 2 (AGR2): blood-based biomarker elevated in metastatic prostate cancer associated with the neuroendocrine phenotype [Pubmed: 22911164]
BMC Cancer. Validation of four candidate pancreatic cancer serological biomarkers that improve the performance of CA19.9 [Pubmed: 24007603]
Clinical Cancer Research Validation of Biomarkers That Complement CA19.9 in Detecting Early Pancreatic Cancer [Pubmed:25239611]
PLoS One AGR3 in Breast Cancer: Prognostic Impact and Suitable Serum-Based Biomarker for Early Cancer Detection [PubMed: 25875093]
Clinical Neurology and Neurosurgery Serum AGR2 as a useful biomarker for pituitary adenomas [S0303846717300045]
Oncotarget Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2) [Pubmed:29937991]
Int J Oncol Anterior gradient protein 2 is a marker of tumor aggressiveness in breast cancer and favors chemotherapy‑induced senescence escape [34913074]
International Journal of Cancer Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer [Pubmed:35689436]
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