不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Asymmetrical Dimethylarginine (ADMA)
N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine
- 编号KSB301Ge11
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围24.69-2,000ng/mL
- 可适灵敏度8.98ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8726 ¥ 14544 ¥ 26179 ¥ 50904 ¥ 87264
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对不对称二甲基精氨酸(ADMA)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
CPB301Ge11 | 不对称二甲基精氨酸(ADMA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPB301Ge21 | 不对称二甲基精氨酸(ADMA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAB301Ge01 | 不对称二甲基精氨酸(ADMA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAB301Ge71 | 不对称二甲基精氨酸(ADMA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
CEB301Ge | 不对称二甲基精氨酸(ADMA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB301Ge | 不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB301Ge11 | 不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
PLoS One. | Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in Highlanders [PubMed: PMC3439466] |
Cytokine | Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats. [Pubmed: 23834755] |
Neurol Res. | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats. [Pubmed:25475507] |
Int J Clin Exp Med. | Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients [Pubmed:25550968] |
Clin Respir J | Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD [PubMed: 26076870] |
Cytokine | Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive rats [PubMed: 26375520] |
Neurological Research | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats [PubMed: 25475507] |
Scientific Reports | Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study [pubmed:27677852] |
clinica chimica acta | Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease. [pubmed:27884754] |
The Journal of Physiology | Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans. [pubmed:26840628] |
Angiogenesis | Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA). [pubmed:29150732] |
Scientific Reports | A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling [Pubmed:29523851] |
Mechanisms of Ageing and Development | Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territories [Pubmed:29605639] |
Nutrition, Metabolism and Cardiovascular Diseases | Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns [Doi: 10.1016/j.numecd.2018.09.002] |
Biochemical and Biophysical Research Communications | Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats [Pubmed: 30454888] |
Brazilian Journal of Pharmaceutical Science | Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients [] |
ACS Combinatorial Science | Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathway [Pubmed: 30673277] |
International Journal of the Cardiovascular Academy | Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats [] |
Oxid Med Cell Longev | The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT Cells [Pubmed: 32104540] |
Molecular Medicine Reports | Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in … [Pubmed: 31746378] |
International Journal of Nephrology | Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis Patients [Pubmed: 33163233] |
Journal of Clinical Medicine | The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial [Pubmed: 32344735] |
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub | Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls [33463628] |
WIENER KLINISCHE WOCHENSCHRIFT | Non-functioning adrenal incidentalomas may increase toxic metabolites [34255169] |
pharmaceuticals | Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats—A Comparison with Mesna [34959638] |
Int J Biochem Cell Biol | HIF-1α and Nrf2 regulates hypoxia induced overexpression of DDAH1 through promoter activation in prostate cancer [Pubmed:35644470] |
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