溶血磷脂酰胆碱(LPC)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Lysophosphatidylcholine (LPC)
lysoPC; Lysolecithin; 1-Palmitoyl-sn-Glycero-3-Phosphocholine
- 编号KSK621Ge11
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围246.9-20,000ng/mL
- 可适灵敏度89.3ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8813 ¥ 14688 ¥ 26438 ¥ 51408 ¥ 88128
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对溶血磷脂酰胆碱(LPC)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPK621Ge11 | 溶血磷脂酰胆碱(LPC)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPK621Ge21 | 溶血磷脂酰胆碱(LPC)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAK621Ge01 | 溶血磷脂酰胆碱(LPC)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEK621Ge | 溶血磷脂酰胆碱(LPC)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMK621Ge | 溶血磷脂酰胆碱(LPC)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSK621Ge11 | 溶血磷脂酰胆碱(LPC)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Scientific Reports | High-fat diet-induced acceleration of osteoarthritis is associated with a distinct and sustained plasma metabolite signature [pubmed:28811491] |
| Stem Cells | Iron Homeostasis Determines Fate of Human Pluripotent Stem Cells Via Glycerophospholipids‐Epigenetic Circuit [Pubmed: 30599084] |
| Lipids in Health and Disease | Associations between plasma lysophospholipids concentrations, chronic kidney disease and the type of renal replacement therapy [Pubmed: 30947711] |
| Nature Communications | ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy [Pubmed: 31358770] |
| NUTRIENTS | Dietary Betaine Addition Promotes Hepatic Cholesterol Synthesis, Bile Acid Conversion, and Export in Rats [Pubmed: 32414094] |
| Cells | iPLA2¦Â Contributes to ER Stress-Induced Apoptosis during Myocardial Ischemia/Reperfusion Injury [] |
| European Journal of Pharmacology | Downregulation of activating transcription factor 4 attenuates lysophosphatidycholine-induced inflammation via the NF-κB pathway [34582847] |
| Oxid Med Cell Longev | Astragaloside IV Inhibits Bleomycin-Induced Ferroptosis in Human Umbilical Vein Endothelial Cells by Mediating LPC [34760046] |
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