抗增殖细胞核抗原(PCNA)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Proliferating Cell Nuclear Antigen (PCNA)

Cyclin

特异性

试剂盒中的抗体对抗增殖细胞核抗原(PCNA)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
URPA591Hu01 抗增殖细胞核抗原(PCNA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UCAA591Hu01 抗抗增殖细胞核抗原(PCNA)多克隆抗体 Loading Control of WB.
UPAA591Hu01 抗增殖细胞核抗原(PCNA)多克隆抗体 WB; IHC; ICC; IP.
UMAA591Hu21 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
UMAA591Hu22 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
UMAA591Hu23 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
UMAA591Hu25 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
UMAA591Hu24 抗增殖细胞核抗原(PCNA)单克隆抗体 WB; IHC; ICC; IP.
UCAA591Hu22 抗抗增殖细胞核抗原(PCNA)单克隆抗体 Loading Control of WB.
UFAA591Hu02 抗抗增殖细胞核抗原(PCNA)单克隆抗体 FCM
UFAA591Hu04 抗抗增殖细胞核抗原(PCNA)单克隆抗体 FCM
UKSA591Hu01 抗增殖细胞核抗原(PCNA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … []
Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation … []
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