95 KSP155Hu01 | 无翅型MMTV整合位点家族成员3A(WNT3A)检测试剂盒DIY材料(酶联免疫吸附试验法) | Homo sapiens (Human,人) USCN(武汉优尔生商贸有限公司)

无翅型MMTV整合位点家族成员3A(WNT3A)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)

  • 无翅型MMTV整合位点家族成员3A(WNT3A)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 无翅型MMTV整合位点家族成员3A(WNT3A)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对无翅型MMTV整合位点家族成员3A(WNT3A)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

参考文献

杂志 参考文献
Medical Oncology Wls promotes the proliferation of breast cancer cells via Wnt signaling [Pubmed:25801232]
J Histochem Cytochem Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs [PubMed: 26209081]
PloS one Gelatin device for the delivery of growth factors involved in endochondral ossification [pubmed:28380024]
Life Sciences Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618]
Oxidative Medicine and Cellular Longevity Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus Muscle [Pubmed:30151071]
The Showa University Journal of Medical Sciences Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer [Doi: 10.15369/sujms.30.359]
Journal of Cancer Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma [Pubmed: 31737122]
CANCER LETTERS Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection [Pubmed: 32920199]
Biomaterials An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties [34823885]
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