血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

  • 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA143Ca.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管内皮生长因子A(VEGFA)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管内皮生长因子A(VEGFA)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 88-95 92
EDTA plasma(n=5) 83-93 88
heparin plasma(n=5) 88-102 91

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的血管内皮生长因子A(VEGFA)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管内皮生长因子A(VEGFA)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 79-102% 79-103% 94-103% 79-97%
EDTA plasma(n=5) 89-98% 93-104% 90-98% 83-98%
heparin plasma(n=5) 84-91% 78-88% 78-98% 87-105%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将血管内皮生长因子A(VEGFA)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管内皮生长因子A(VEGFA)与连接于固相载体上的抗体结合，然后加入生物素化的血管内皮生长因子A(VEGFA)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管内皮生长因子A(VEGFA)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Canis familiaris; Canine (Dog，犬) 应用(仅供研究使用，不用于临床诊断！)
RPA143Ca01 血管内皮生长因子A(VEGFA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA143Ca61 血管内皮生长因子A(VEGFA)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA143Ca01 血管内皮生长因子A(VEGFA)多克隆抗体 WB; IHC; ICC; IP.
LAA143Ca71 血管内皮生长因子A(VEGFA)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA143Ca21 血管内皮生长因子A(VEGFA)单克隆抗体 WB; IHC; ICC; IP.
SEA143Ca 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA143Ca 血管内皮生长因子A(VEGFA)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA143Ca 血管内皮生长因子A(VEGFA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

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Clinical and Experimental Immunology Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients [Pubmed:35348620]
Medicina Assessment of the RANTES Level Correlation and Selected Inflammatory and Pro-Angiogenic Molecules Evaluation of Their Influence on CRC Clinical Features: A … [Pubmed:35208526]
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377]
Biomaterials Advances Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats []
Biointerface Research in Applied Chemistry Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats []
Research Square DMOG attenuates steroid-associated endothelial progenitor cell impairment and osteonecrosis of the femoral head via regulating HIF-1α signaling pathway in … []
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