组胺(HA)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Histamine (HA)
Histamin
- 编号UCEA927Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围390.6-100,000pg/mL
- 灵敏度最小可检测剂量小于等于137.3pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 1671 ¥ 2387 ¥ 10741 ¥ 20288 ¥ 167076
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测组胺(HA)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的组胺(HA)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 92-105 | 101 |
| EDTA plasma(n=5) | 97-105 | 102 |
| heparin plasma(n=5) | 80-103 | 91 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的组胺(HA)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中组胺(HA)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-99% | 99-105% | 98-105% | 93-103% |
| EDTA plasma(n=5) | 91-101% | 91-105% | 95-102% | 91-101% |
| heparin plasma(n=5) | 95-104% | 90-99% | 93-101% | 89-103% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将组胺抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原（标准品或样本），待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| UCPA927Ge41 | 组胺(HA)人血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| UCPA927Ge11 | 组胺(HA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| UCPA927Ge21 | 组胺(HA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| UPAA927Ge01 | 组胺(HA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAA927Ge71 | 组胺(HA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| ULAA927Ge81 | 组胺(HA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| UMAA927Ge21 | 组胺(HA)单克隆抗体 | ELISA;IHC |
| UCEA927Ge | 组胺(HA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA927Ge | 组胺(HA)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA927Ge | 组胺(HA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| UKSA927Ge11 | 组胺(HA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It(ELISA Kit) Yourself" |
参考文献
| 杂志 | 参考文献 |
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| Neurogastroenterology & Motility | Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea [Pubmed:26940641] |
| Italian Journal of Animal Science | Induction of immune responses and allergic reactions in piglets by injecting glycinin [doi:1828051X.2016.1144488] |
| Int J Clin Exp Pathol | Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism [files:ijcep0019305.pdf] |
| PLoS One. | Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbumin [pmc:PMC4734762] |
| Int Immunopharmacol. | Evaluation of anaphylactoid constituents in vitro and in vivo. [pubmed:27984711] |
| Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
| Royal Society Open Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
| International Journal of Pharmaceutical Sciences and Research | MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL [] |
| Allergy | Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial [Pubmed: 32030780] |
| MOLECULAR IMMUNOLOGY | Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806] |
| bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
| Nature Communications | A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy [Pubmed: 32332752] |
| CCR3‑shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells [Pubmed: 32742345] | |
| ANALYTICA CHIMICA ACTA | Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of … [Pubmed: 32892931] |
| Annals of Tropical Medicine & Public Health | Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq [] |
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