中性粒细胞弹性蛋白酶(NE)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Neutrophil Elastase (NE)

ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase

  • 中性粒细胞弹性蛋白酶(NE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 中性粒细胞弹性蛋白酶(NE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 中性粒细胞弹性蛋白酶(NE)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA181Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测中性粒细胞弹性蛋白酶(NE)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的中性粒细胞弹性蛋白酶(NE)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 85-103 93
EDTA plasma(n=5) 89-97 94
heparin plasma(n=5) 87-94 90

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的中性粒细胞弹性蛋白酶(NE)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中中性粒细胞弹性蛋白酶(NE)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 92-101% 87-95% 84-91%
EDTA plasma(n=5) 95-105% 85-98% 88-103% 85-94%
heparin plasma(n=5) 80-88% 89-101% 89-96% 96-103%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将中性粒细胞弹性蛋白酶(NE)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的中性粒细胞弹性蛋白酶(NE)与连接于固相载体上的抗体结合，然后加入生物素化的中性粒细胞弹性蛋白酶(NE)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的中性粒细胞弹性蛋白酶(NE)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
UAPA181Ra01 中性粒细胞弹性蛋白酶(NE)活性蛋白 Cell culture; Activity Assays.
URPA181Ra01 中性粒细胞弹性蛋白酶(NE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAA181Ra01 中性粒细胞弹性蛋白酶(NE)多克隆抗体 WB
UMAA181Ra21 中性粒细胞弹性蛋白酶(NE)单克隆抗体 WB; IHC; ICC; IP.
USEA181Ra 中性粒细胞弹性蛋白酶(NE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA181Ra 中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Mediators of Inflammation Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation [Hindawi: 612848]
Journal of Photochemistry and Photobiology B: Biology A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins [Pubmed:25732262]
International Journal of Molecular Medicine The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats [pubmed:27430552]
European Journal of Trauma and Emergency Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. [pubmed:28070608]
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. [pubmed:28251688]
Journal of Cardiology Targeted regulation of sympathetic activity in paraventricular nucleus reduces inducible ventricular arrhythmias in rats after myocardial infarction [10.1016:j.jjcc.2018.06.003]
Neuroscience Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats [Pubmed:29684507]
Molecular and Cellular Biochemistry Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes [Pubmed:28948423]
Neuropsychiatric disease and treatment The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … [Pubmed:29379286]
Behavioural Brain Research Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats [Pubmed: 30423388]
Journal of Pineal Research Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267]
Fundamental & clinical pharmacology Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo [Pubmed: 31313350]
Cell Death & Disease Erythromycin suppresses neutrophil extracellular traps in smoking-related chronic pulmonary inflammation [Pubmed: 31515489]
Journal of nutritional science and vitaminology Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively … [Pubmed: 31474682]
Journal of Clinical Immunology MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in Monocytes [Pubmed: 31749032]
Pathology & Oncology Research Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer [Pubmed: 31656990]
Bioscience Reports Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats [Pubmed: 31763669]
Oxidative Medicine and Cellular Longevity Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation [Pubmed: 31871555]
Cytokine Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis [Pubmed: 31927460]
Journal of neural transmission (Vienna, Austria : 1996) Neurotransmitter system aberrations in patients with drug addiction [Pubmed: 32804296]
experimental eye research Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis [Pubmed: 32376471]
CLINICAL AND EXPERIMENTAL HYPERTENSION Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats [Pubmed: 32349626]
Br J Pharmacol Macrophage Extracellular Traps Aggravate Iron Overload©\Related Liver Ischemia/Reperfusion Injury [33959955]
Evidence-Based Complementary and Alternative Medicine Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡­ [33505502]
Nat Sci Sleep Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with … [34934375]
Neuromodulation: Technology at the Neural Interface Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome [Pubmed:35088760]
A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation []
留言咨询